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Resumen
Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study,
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dc.contributor.author | de Francesco, Agustina | |
dc.contributor.author | Costa, Norma Beatriz | |
dc.contributor.author | Garcia, Maria Laura | |
dc.date.accessioned | 2018-07-20T15:44:03Z | |
dc.date.available | 2018-07-20T15:44:03Z | |
dc.date.issued | 2017-04 | |
dc.identifier.issn | 0962-8819 | |
dc.identifier.issn | 1573-9368 | |
dc.identifier.other | https://doi.org/10.1007/s11248-016-0001-2 | |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs11248-016-0001-2 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/2843 | |
dc.description.abstract | Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation. In particular, line ihpCP-15 has been resistant for more than 2 years, even after re-inoculation. The ihpCP plants were also resistant against a heterologous CPsV isolate that causes severe PsB syndrome. Line ihpCP-15 manifested complete resistance while line ihpCP-10 was tolerant to the virus, although with variable behaviour, showing delay and attenuation in PsB symptoms. These lines are promising for a biotech product aimed at eradicating psorosis. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Transgenic Research 26 (2) : 225–235 (April 2017) | es_AR |
dc.subject | Citrus | es_AR |
dc.subject | Virus de las Plantas | es_AR |
dc.subject | Plant Viruses | eng |
dc.subject | Plantas Transgénicas | es_AR |
dc.subject | Transgenic Plants | eng |
dc.subject | Resistencia a la Enfermedad | es_AR |
dc.subject | Disease Resistance | eng |
dc.subject.other | Citrus Psorosis Virus | es_AR |
dc.subject.other | Virus de la Psoriasis de los Cítricos | es_AR |
dc.title | Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | EEA Concordia | es_AR |
dc.description.fil | Fil: de Francesco, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Costa, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina | es_AR |
dc.description.fil | Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina | es_AR |
dc.subtype | cientifico |
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