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resumen

Resumen
Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, [ver mas...]
dc.contributor.authorde Francesco, Agustina
dc.contributor.authorCosta, Norma Beatriz
dc.contributor.authorGarcia, Maria Laura
dc.date.accessioned2018-07-20T15:44:03Z
dc.date.available2018-07-20T15:44:03Z
dc.date.issued2017-04
dc.identifier.issn0962-8819
dc.identifier.issn1573-9368
dc.identifier.otherhttps://doi.org/10.1007/s11248-016-0001-2
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs11248-016-0001-2
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2843
dc.description.abstractCitrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation. In particular, line ihpCP-15 has been resistant for more than 2 years, even after re-inoculation. The ihpCP plants were also resistant against a heterologous CPsV isolate that causes severe PsB syndrome. Line ihpCP-15 manifested complete resistance while line ihpCP-10 was tolerant to the virus, although with variable behaviour, showing delay and attenuation in PsB symptoms. These lines are promising for a biotech product aimed at eradicating psorosis.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceTransgenic Research 26 (2) : 225–235 (April 2017)es_AR
dc.subjectCitruses_AR
dc.subjectVirus de las Plantases_AR
dc.subjectPlant Viruseseng
dc.subjectPlantas Transgénicases_AR
dc.subjectTransgenic Plantseng
dc.subjectResistencia a la Enfermedades_AR
dc.subjectDisease Resistanceeng
dc.subject.otherCitrus Psorosis Viruses_AR
dc.subject.otherVirus de la Psoriasis de los Cítricoses_AR
dc.titleCitrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromeses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Concordiaes_AR
dc.description.filFil: de Francesco, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Costa, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentinaes_AR
dc.description.filFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentinaes_AR
dc.subtypecientifico


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