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Resumen
Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants
were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to
expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they
showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection without the
recovery observed in non-transgenic plants.
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dc.contributor.author | Reyes Martinez, Carina Andrea | |
dc.contributor.author | de Francesco, Agustina | |
dc.contributor.author | Ocolotobiche, Eliana Evelina | |
dc.contributor.author | Costa, Norma Beatriz | |
dc.contributor.author | Garcia, Maria Laura | |
dc.date.accessioned | 2018-07-19T14:20:28Z | |
dc.date.available | 2018-07-19T14:20:28Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0885-5765 | |
dc.identifier.other | https://doi.org/10.1016/j.pmpp.2016.05.001 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/2827 | |
dc.description.abstract | Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection without the recovery observed in non-transgenic plants. Ihp24K lines did not behave similarly when challenged with Citrus tristeza virus. All these results indicate that hypersusceptibility is likely related to the specific action of 24K-derived hairpin over CPsV multiplication. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.rights | info:eu-repo/semantics/restrictedAccess | eng |
dc.source | Physiological and molecular plant pathology 94 : 149-155. (2016) | eng |
dc.subject | Citrus Sinensis | es_AR |
dc.subject | Infección | es_AR |
dc.subject | Virus de las Plantas | es_AR |
dc.subject | Plantas Transgénicas | es_AR |
dc.subject | Naranja Dulce | es_AR |
dc.subject | ARN | es_AR |
dc.subject | RNA | eng |
dc.subject | Sweet Oranges | eng |
dc.subject | Transgenic Plants | eng |
dc.subject | Plant Viruses | eng |
dc.subject | Infection | eng |
dc.subject | Citrus Sinensis | eng |
dc.subject.other | Citrus Psorosis Virus | eng |
dc.subject.other | Virus de la Psoriasis de los Cítricos | es_AR |
dc.subject.other | Hypersusceptibility | eng |
dc.subject.other | Hairpin-RNA | eng |
dc.title | Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.description.origen | EEA Concordia | es_AR |
dc.description.fil | Fil: Reyes Martinez, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: de Francesco, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Ocolotobiche, Eliana Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Costa, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina | es_AR |
dc.description.fil | Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina | es_AR |
dc.subtype | cientifico |
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