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Abstract
Interferon-γ (IFN-γ) recall responses against foot-and-mouth disease virus (FMDV) in FMD vaccinated cattle are utilized to study T-lymphocyte immunity against this virus. Here, a recall IFN-γ assay based on a commercial ELISA was set up using 308 samples from naïve and vaccinated cattle. The assay was used to study cross-reactive responses between different FMDV vaccine strains. Blood samples from cattle immunized with monovalent vaccines containing [ver mas...]
dc.contributor.authorBucafusco, Danilo
dc.contributor.authorDi Giacomo, Sebastián
dc.contributor.authorPega, Juan Franco
dc.contributor.authorSchammas, Juan Manuel
dc.contributor.authorCardoso, Nancy Patricia
dc.contributor.authorCapozzo, Alejandra Victoria
dc.contributor.authorPerez Filgueira, Daniel Mariano
dc.date.accessioned2018-05-30T14:14:43Z
dc.date.available2018-05-30T14:14:43Z
dc.date.issued2015-02
dc.identifier.issn0042-6822
dc.identifier.otherhttps://doi.org/10.1016/j.virol.2014.11.023
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0042682214005315
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2516
dc.description.abstractInterferon-γ (IFN-γ) recall responses against foot-and-mouth disease virus (FMDV) in FMD vaccinated cattle are utilized to study T-lymphocyte immunity against this virus. Here, a recall IFN-γ assay based on a commercial ELISA was set up using 308 samples from naïve and vaccinated cattle. The assay was used to study cross-reactive responses between different FMDV vaccine strains. Blood samples from cattle immunized with monovalent vaccines containing A24/Cruzeiro/Brazil/55, A/Argentina/2001 or O1/Campos/Brazil/58 strains were tested using purified-inactivated FMDV from homologous and heterologous strains. A24/Cruzeiro was the most efficient IFN-γ inducer in all vaccinated animals, both when included in the vaccine or as stimulating antigen. We demonstrate that this was mainly due to the structural stability of the whole viral particle. These results show that IFN-γ production relies on the presence of 140S particles that can maintain their integrity along the incubation process in vitro, and throughout the vaccine´s shelf-life, when used in vivo.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceVirology 476 : 11-18 (February 2015)es_AR
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectFiebre Aftosaes_AR
dc.subjectFoot and Mouth Diseaseeng
dc.subjectVacunaciónes_AR
dc.subjectVaccinationeng
dc.subjectInterferonases_AR
dc.subjectInterferonseng
dc.titleFoot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particleses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Bucafusco, Danilo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Schammas, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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