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Abstract
Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. According to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV1-related virus infection in [ver mas...]
dc.contributor.authorMaidana, Silvina Soledad
dc.contributor.authorKonrad, Jose Luis
dc.contributor.authorCraig, Marí­a Isabel
dc.contributor.authorZabal, Osvaldo Alfredo
dc.contributor.authorMauroy, Axel
dc.contributor.authorThiry, Etienne
dc.contributor.authorCrudeli, Gustavo Angel
dc.contributor.authorRomera, Sonia Alejandra
dc.date.accessioned2018-05-14T15:19:15Z
dc.date.available2018-05-14T15:19:15Z
dc.date.issued2014-11
dc.identifier.issn0304-8608
dc.identifier.issn1432-8798
dc.identifier.otherhttps://doi.org/10.1007/s00705-014-2146-8
dc.identifier.urihttps://link.springer.com/article/10.1007/s00705-014-2146-8
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2393
dc.description.abstractHerpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. According to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV1-related virus infection in wild and domestic ruminant species. Recent studies in Argentina showed an increase in serological prevalence against BoHV1 related viruses in water buffaloes (Bubalus bubalis) population. The aim of this study was to investigate the presence of related ruminant alphaherpesvirus in the Argentinean water buffalo population. BuHV1 was successfully isolated from 5 out of 225 buffaloes analyzed. One isolate was obtained from nasal secretions, and the others were from vaginal swabs. The buffaloes belonged to four different farms located in northeastern Argentina. The isolates were characterized as alphaherpesvirus by direct immunofluorescence using FITC-anti-BoHV1 IgG. Restriction analysis performed with BamHI and BstEII on the complete genome showed differences between the isolates and those from BoHV1 and BoHV5 subtypes. Phylogenetic analysis on both UL27 and US6 showed similarity in tree topology. While three of the isolates grouped together with sequences of BoHV5, two other isolates clustered separately. Genetic analysis of eight concatenated sequences from all isolates and references strains showed high nucleotide sequence identity between BuHV1 and BoHV5. While three of the isolates clustered together with the BoHV5 reference strain, the last two isolates were closely related to an Australian BuHV1 strain. To our knowledge, this is the first report on the isolation and molecular characterization of BuHV1 in South America. Phylogenetic analysis suggested that two different BuHV1 lineages circulate in the Argentinean water buffalo population.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceArchives of Virology 159 (11) : 2917–2923. (November 2014)es_AR
dc.subjectBúfalo de Aguaes_AR
dc.subjectWater Buffaloeseng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectHerpesviridaees_AR
dc.subjectTécnicas de Aislamientoes_AR
dc.subjectIsolation Techniqueseng
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subject.otherHerpesviruses_AR
dc.subject.otherArgentinaes_AR
dc.titleFirst report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloeses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentinaes_AR
dc.description.filFil: Konrad, Jose Luis. Universidad Nacional del Nordeste. Facultad de Veterinaria. Cátedra de Teriogenologia; Argentinaes_AR
dc.description.filFil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Mauroy, Axel. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgicaes_AR
dc.description.filFil: Thiry, Etienne. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgicaes_AR
dc.description.filFil: Crudeli, Gustavo Angel. Universidad Nacional del Nordeste. Facultad de Veterinaria. Cátedra de Teriogenologia; Argentinaes_AR
dc.description.filFil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentinaes_AR
dc.subtypecientifico


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