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Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum

Abstract
Plant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic tobacco expressing a variant of Tobacco mosaic virus (TMV) coat protein (CPT42W) or movement protein (MP), and a hybrid line (MP×CPT42W) that coexpresses both proteins, to study the plant response to individual viral proteins. Findings [ver mas...]
Plant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic tobacco expressing a variant of Tobacco mosaic virus (TMV) coat protein (CPT42W) or movement protein (MP), and a hybrid line (MP×CPT42W) that coexpresses both proteins, to study the plant response to individual viral proteins. Findings employing microarray analysis of MP×CPT42W plants and silenced mp×cpT42W* controls revealed that altered transcripts were mostly downregulated, suggesting a persistent shut-off due to MP×CPT42W expression. Next, we showed that MP triggered reactive oxygen species (ROS) accumulation, reduction of total ascorbate, and expression of ROS scavenging genes. These effects were enhanced when both proteins were coexpressed. MP and MP×CPT42W plants showed increased levels of salicylic acid (SA) and SA-responsive gene expression. Furthermore, these effects were partially reproduced in Nicotiana benthamiana when GMP1 transcript was silenced. CPT42W seems to be playing a negative role in the defense response by reducing the expression of PR-1 and RDR-1. MP and MP×CPT42W transgenic expression promoted a recoverylike phenotype in TMV RNA infections and enhanced susceptibility to Pseudomonas syringae and Sclerotinia sclerotiorum. The individual effects of viral proteins may reflect the ability of a virus to balance its own virulence. [Cerrar]
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Author
Conti, Gabriela;   Rodriguez, Maria Cecilia;   Manacorda, Carlos Augusto;   Asurmendi, Sebastian;  
Fuente
Molecular plant-microbe interactions 25 (10) : 1370–1384. (2012)
Date
2012
ISSN
0894-0282
1943-7706 (online version)
URI
http://hdl.handle.net/20.500.12123/2181
https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-03-12-0075-R
DOI
https://doi.org/10.1094/MPMI-03-12-0075-R
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Nicotiana Tabacum; Tabaco; Proteínas Virales; Estrés Biótico; Virus de las Plantas; ARN; RNA; Plant Viruses; Tobacco; Tobacco Mosaic Virus;
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Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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