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Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes

Abstract
Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the [ver mas...]
Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration. [Cerrar]
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Author
Bazzini, Ariel Alejandro;   Manacorda, Carlos Augusto;   Tohge, Takayuki;   Conti, Gabriela;   Rodriguez, Maria Cecilia;   Nunes Nesi, Adriano;   Villanueva, Sofía;   Fernie, Alisdair R.;   Carrari, Fernando;   Asurmendi, Sebastian;  
Fuente
PLoS ONE 6 (12) :e28466. (December 2011)
Date
2011-12-12
ISSN
1932-6203
URI
http://hdl.handle.net/20.500.12123/2168
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236191/
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pdf
Tipo de documento
artículo
Palabras Claves
RNA; Metabolismo; Virus de las Plantas; Nicotiana Tabacum; Tabaco; Cromatografía Gas-líquido; Espectrometría de Masas; Mass Spectrometry; Gas Liquid Chromatography; Tobacco; Plant Viruses; Virus del Mosaico del Tabaco; miRNA;
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Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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