Show simple item record

resumen

Abstract
Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [ver mas...]
dc.contributor.authorSoria, Ivana
dc.contributor.authorQuattrocchi, Valeria
dc.contributor.authorLangellotti, Cecilia Ana
dc.contributor.authorGammella, Mariela
dc.contributor.authorDi Giacomo, Sebastián
dc.contributor.authorGarcia de la Torre, Beatriz
dc.contributor.authorAndreu, David
dc.contributor.authorMontoya, Maria
dc.contributor.authorSobrino, Francisco
dc.contributor.authorBlanco, Esther
dc.contributor.authorZamorano, Patricia Ines
dc.date.accessioned2018-03-09T15:54:33Z
dc.date.available2018-03-09T15:54:33Z
dc.date.issued2017-09
dc.identifier.issn1932-6203
dc.identifier.otherhttps://doi.org/10.1371/journal.pone.0185184
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2006
dc.identifier.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185184
dc.description.abstractFoot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourcePLoS ONE 12 (9) : e0185184 (2017)es_AR
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectVirus de los Animaleses_AR
dc.subjectAnimal Viruseseng
dc.subjectGanado Bovinoes_AR
dc.subjectCattleeng
dc.subjectFiebre Aftosaes_AR
dc.subjectFoot and Mouth Diseaseeng
dc.subjectPéptidoses_AR
dc.subjectPeptideseng
dc.titleDendrimeric peptides can confer protection against foot-and-mouth disease virus in cattlees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentinaes_AR
dc.description.filFil: Garcia de la Torre, Beatriz. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; Españaes_AR
dc.description.filFil: Andreu, David. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; Españaes_AR
dc.description.filFil: Montoya, María. Pirbright Institute; Gran Bretañaes_AR
dc.description.filFil: Sobrino, Francisco. Universidad Autónoma de Madrid. Centro de Biología Molecular “Severo Ochoa"; Españaes_AR
dc.description.filFil: Blanco, Esther. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Centro de Investigación en Sanidad Animal; Españaes_AR
dc.description.filFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record