Show simple item record

resumen

Abstract
Closely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as [ver mas...]
dc.contributor.authorMaidana, Silvina Soledad
dc.contributor.authorCraig, Patricio Oliver
dc.contributor.authorCraig, María Isabel
dc.contributor.authorLudwig, Louisa
dc.contributor.authorMauroy, Axel
dc.contributor.authorThiry, Etienne
dc.contributor.authorRomera, Sonia Alejandra
dc.date.accessioned2018-03-09T11:25:58Z
dc.date.available2018-03-09T11:25:58Z
dc.date.issued2017-10
dc.identifier.issn0168-1702
dc.identifier.otherhttps://doi.org/10.1016/j.virusres.2017.09.018
dc.identifier.urihttp://hdl.handle.net/20.500.12123/2000
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0168170217302976?via%3Dihub
dc.description.abstractClosely related bovine alphaherpesviruses 1 (BoHV-1) and 5 (BoHV-5) co-circulate in certain countries, rendering cattle co-infection possible. This is a prerequisite for BoHV recombination. Here, we report the first identification of homologous recombination between field isolates of BoHV-1 and BoHV-5, two alphaherpesviruses belonging to two distinct species with an average genomic similarity of 82.3%. Three isolates of BoHV-5, previously classified as subtype “BoHV-5b”, were phylogenetically studied and analyzed via eight PCR sequencing assays dispersed at regular intervals throughout the genome to discriminate between BoHV-1 andBoHV-5. In the phylogenetic analysis, differences of clustering were found in the UL27 gene which encodes the glycoprotein B (gB). We detected two recombination breakpoints in the open reading frame of the UL27 gene. We compared the amino acid sequences of the gB of BoHV-1.1 and 1.2, BoHV-5a and recombinant formerly named BoHV-5b (chimeric gB) and subsequently performed molecular modeling. All structures were alike and, simultaneously, similar to the chimeric gB. Neutralizing antibodies against BoHV-1, BoHV-5 and recombinant viruses were analyzed via serum virus neutralization test using polyclonal sera and a monoclonal antibody against gB to demonstrate an absence of viral escape for both assays. Our results show that homologous recombination between two related species of ruminant alphaherpesviruses can occur in natural field conditions. We found three recombinant field isolates, previously classified as BoHV-5b subtypes, between BoHV-1 and BoHV-5.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceVirus research 242 : 122-130. (October 2017)
dc.subjectGanado Bovino
dc.subjectCattleeng
dc.subjectEnfermedades de los Animales
dc.subjectAnimal Diseaseseng
dc.subjectHerpesviridae
dc.subjectHerpes Virus Bovino
dc.subjectBovine Herpesviruseng
dc.subject.otherHerpesvirus
dc.titleEvidence of natural interspecific recombinant viruses between bovine alphaherpesviruses 1 and 5eng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInst.de Virología
dc.gic155608
dc.description.filFil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina
dc.description.filFil: Craig, Patricio Oliver. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Biológica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
dc.description.filFil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Ludwig, Louisa. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica
dc.description.filFil: Mauroy, Axel. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica
dc.description.filFil: Thiry, Etienne. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica
dc.description.filFil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record