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Aplicaciones y perspectivas de la edición génica de plantas mediante CRISPR-Cas9 = Applications and perspectives of plant gene editing using CRISPR-Cas9
(Universidad de Morón, 2022)Los avances en materia de genética y biotecnología, introducen una dimensión novedosa en la utilización de herramientas de edición génica en plantas. En el presente trabajo se relevaron 164 publicaciones científicas que ... -
Baculovirus transduction in mammalian cells is affected by the production of type I and III interferons, which is mediated mainly by the cGAS-STING pathway
(American Society for Microbiology, 2020-11)The baculovirus Autographa californica multiple nucleopolyhedrovirus is an insect virus with a circular double-stranded DNA genome, which, among other multiple biotechnological applications, is used as an expression vector ... -
Balance between retroviral latency and transcription : based on HIV model
(MDPI, 2021-01)The representative of the Lentivirus genus is the human immunodeficiency virus type 1 (HIV-1), the causative agent of acquired immunodeficiency syndrome (AIDS). To date, there is no cure for AIDS because of the existence ... -
Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies
(Frontiers Media, 2021-11)Citrus are among the most prevailing fruit crops produced worldwide. The implementation of effective and reliable breeding programs is essential for coping with the increasing demands of satisfactory yield and quality of ... -
CRISPR/Cas9 and genetic screens in malaria parasites : small genomes, big impact
(Portland Press, 2022-06)The ∼30 Mb genomes of the Plasmodium parasites that cause malaria each encode ∼5000 genes, but the functions of the majority remain unknown. This is due to a paucity of functional annotation from sequence homology, which ... -
CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa
(Springer, 2023-02)Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying ... -
CRISPR/Cas9‑mediated knockout of SPL13 radically increases lettuce yield
(Springer, 2022-12)Cultivated lettuce (Lactuca sativa L.) is a diploid (2n = 2x = 18), autogamous species. This popular leafy vegetable is considered one of the first crops (Guo et al. 2022). Currently, lettuce is the third most consumed ... -
Efficient CRISPR/Cas9 genome editing in alfalfa using a public germplasm
(Frontiers Media, 2021-07)Because its ability to acquire large amounts of nitrogen by symbiosis, tetraploid alfalfa is the main source of vegetable proteins in meat and milk production systems in temperate regions. Alfalfa cultivation also adds ... -
Evaluation of a lyophilized CRISPR-Cas12 assay for a sensitive, specific, and rapid detection of SARS-CoV-2
(MDPI, 2021-03)We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted ... -
Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa
(Frontiers Media, 2022-04)Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research ... -
Generation of a multi‑herbicide‑tolerant alfalfa by using base editing
(Springer, 2022-02)We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important ... -
Multiple challenges in the development of commercial crops using CRISPR technology
(Elsevier, 2023-10)The CRISPR/Cas system is a highly efficient and versatile tool for editing plant genomes, with the potential to accelerate breeding programs and improve the sustainability of food production. Nevertheless, technical ... -
Next-generation technologies and systems biology for the design of novel vaccines against apicomplexan parasites
(Frontiers Media, 2022-01)Parasites of the phylum Apicomplexa are the causative agents of important diseases such as malaria, toxoplasmosis or cryptosporidiosis in humans, and babesiosis and coccidiosis in animals. Whereas the first human recombinant ... -
Rapid and cloning‑free screening of edited alfalfa via next‑generation sequencing
(Springer, 2022-07)The identification of full knockout plants is frequently the rate-limiting step of the research and commercial projects searching the complete loss of the function of target genes via the CRISPR/Cas9 system. This problem ... -
Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
(Springer, 2022-07)The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities ... -
The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa
(Springer, 2022-12)The CRISPR/Cas9 system has proven to be highly valuable for genome editing in several important crops, including the allogamous tetraploid cultivated alfalfa (Medicago sativa). However, the fact that most of the beneficial ...