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Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa

Abstract
Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic [ver mas...]
Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research is the search of systems to identify dominant mutations. In this article, we show that the Tnt1 element can be used to identify dominant mutations in allogamous tetraploid cultivated alfalfa. Specifically, we show that a single allelic mutation in the MsNAC39 gene produces multifoliate leaves (mfl) alfalfa plants, a pivot trait of breeding programs of this forage species. Finally, we discuss the potential application of a combination of preliminary screening of beneficial dominant mutants using Tnt1 mutant libraries and genome editing via the CRISPR/Cas9 system to identify target genes and to rapidly improve both autogamous and allogamous polyploid crops. [Cerrar]
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Author
Jozefkowicz, Cintia;   Gomez, Maria Cristina;   Odorizzi, Ariel;   Iantcheva, Anelia;   Ratet, Pascal;   Ayub, Nicolás Daniel;   Soto, Gabriela Cynthia;  
Fuente
Frontiers in Plant Science 12 : 805032. (December 2021)
Date
2022-04
Editorial
Frontiers Media
ISSN
1664-462X
URI
http://hdl.handle.net/20.500.12123/12065
https://www.frontiersin.org/articles/10.3389/fpls.2021.805032/full
DOI
https://doi.org/10.3389/fpls.2021.805032
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INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario

Palabras Claves
Genetic Transformation; Transformación Genética; Polyploidy; Poliploidia; Medicago sativa; Mutation Breeding; Mejoramiento por Mutación; Gene Editing; Edición de Genes; CRISPR; Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas;
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Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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