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Equine rotavirus A (ERVA) is the leading cause of diarrhea in foals, with G3P[12] and G14P[12] genotypes being the most prevalent. Recently, equine G3-like RVA was recognized as an emerging infection in children, and a group B equine rotavirus (ERVB) was identified as an emergent cause of foal diarrhea in the US. Thus, there is a need to adapt molecular diagnostic tools for improved detection and surveillance to identify emerging strains, understand their
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dc.contributor.author | Carossino, Mariano | |
dc.contributor.author | Balasuriya, Udeni B.R. | |
dc.contributor.author | Thieulent, Côme J. | |
dc.contributor.author | Barrandeguy, Maria Edith | |
dc.contributor.author | Vissani, Maria Aldana | |
dc.contributor.author | Parreño, Gladys Viviana | |
dc.date.accessioned | 2023-09-14T10:35:36Z | |
dc.date.available | 2023-09-14T10:35:36Z | |
dc.date.issued | 2023-07 | |
dc.identifier.issn | 1999-4915 | |
dc.identifier.other | https://doi.org/10.3390/v15081626 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/15205 | |
dc.identifier.uri | https://www.mdpi.com/1999-4915/15/8/1626 | |
dc.description.abstract | Equine rotavirus A (ERVA) is the leading cause of diarrhea in foals, with G3P[12] and G14P[12] genotypes being the most prevalent. Recently, equine G3-like RVA was recognized as an emerging infection in children, and a group B equine rotavirus (ERVB) was identified as an emergent cause of foal diarrhea in the US. Thus, there is a need to adapt molecular diagnostic tools for improved detection and surveillance to identify emerging strains, understand their molecular epidemiology, and inform future vaccine development. We developed a quadruplex TaqMan® RT-qPCR assay for differentiation of ERVA and ERVB and simultaneous G-typing of ERVA strains, evaluated its analytical and clinical performance, and compared it to (1) a previously established ERVA triplex RT-qPCR assay and (2) standard RT-PCR assay and Sanger sequencing of PCR products. This quadruplex RT-qPCR assay demonstrated high sensitivity (>90%)/specificity (100%) for every target and high overall agreement (>96%). Comparison between the triplex and quadruplex assays revealed only a slightly higher sensitivity for the ERVA NSP3 target using the triplex format (p-value 0.008) while no significant differences were detected for other targets. This quadruplex RT-qPCR assay will significantly enhance rapid surveillance of both ERVA and ERVB circulating and emerging strains with potential for interspecies transmission. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | MDPI | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | es_AR |
dc.source | Viruses 15 (8) : 1626 (Agosto 2023) | es_AR |
dc.subject | Rotavirus | eng |
dc.subject | Diarrhoea | eng |
dc.subject | Diarrea | es_AR |
dc.subject | PCR | eng |
dc.subject | Equidae | eng |
dc.subject | Genotypes | eng |
dc.subject | Genotipos | es_AR |
dc.subject.other | Equine Rotavirus A | eng |
dc.subject.other | Rotavirus A Equino | es_AR |
dc.subject.other | Equine Rotavirus B | eng |
dc.subject.other | Rotavirus B Equino | es_AR |
dc.title | Quadruplex real-time TaqMan® RT-qPCR assay for differentiation of equine group A and B rotaviruses and Identification of group A G3 and G14 genotypes | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | es_AR |
dc.description.origen | Instituto de Virología | es_AR |
dc.description.fil | Fil: Carossino, Mariano. Louisiana State University. School of Veterinary Medicine. Louisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences; Estados Unidos | es_AR |
dc.description.fil | Fil: Balasuriya, Udeni B. R. Louisiana State University. School of Veterinary Medicine. Louisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences; Estados Unidos | es_AR |
dc.description.fil | Fil: Thieulent, Côme J. Louisiana State University. School of Veterinary Medicine. Louisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences; Estados Unidos | es_AR |
dc.description.fil | Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | es_AR |
dc.description.fil | Fil: Barrandeguy, Maria Edith. Universidad del Salvador. Escuela de Veterinaria; Argentina | es_AR |
dc.description.fil | Fil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología: Argentina | es_AR |
dc.description.fil | Fil: Vissani, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Vissani, Aldana. Universidad del Salvador. Escuela de Veterinaria; Argentina | es_AR |
dc.description.fil | Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | es_AR |
dc.description.fil | Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina | es_AR |
dc.subtype | cientifico |
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