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Resumen
The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we
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dc.contributor.author | Cardoso, Nancy | |
dc.contributor.author | Rivero, Carla | |
dc.contributor.author | Castillo, Mariangeles | |
dc.contributor.author | Mansilla, Florencia Celeste | |
dc.contributor.author | Pastorino, Florencia | |
dc.contributor.author | Piccirilli, Guadalupe | |
dc.contributor.author | Alonso, Laura | |
dc.contributor.author | Martinez, Gustavo | |
dc.contributor.author | Di Lullo, David | |
dc.contributor.author | Bentancor, Leticia Veronica | |
dc.contributor.author | Capozzo, Alejandra | |
dc.coverage.spatial | Greater Buenos Aires .......... (general region) (World, South America, Argentina) | es_AR |
dc.coverage.spatial | 8725264 | es_AR |
dc.date.accessioned | 2023-07-05T17:42:07Z | |
dc.date.available | 2023-07-05T17:42:07Z | |
dc.date.issued | 2023-05 | |
dc.identifier.issn | 2297-1769 | |
dc.identifier.other | https://doi.org/10.3389/fvets.2023.1161820 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/14703 | |
dc.identifier.uri | https://www.frontiersin.org/articles/10.3389/fvets.2023.1161820/full | |
dc.description.abstract | The Coronavirus Disease 2019 (COVID-19) is a zoonotic disease caused by the pandemic virus SARS-CoV-2. Domestic and wild animals are susceptible to infection and are potential reservoirs for virus variants. To date, there is no information about the exposure of companion animals in Buenos Aires Suburbs, the area with the largest population in Argentina where the highest number of COVID-19 human cases occurred during the first infection wave. Here we developed a multi-species indirect ELISA to measure antibodies reactive to the SARS-CoV-2 receptor-binding domain (RBD) from several vertebrates constituting the class Mammalia, making it a valuable tool for field serosurveillance. The ELISA cut-off value was estimated by sera from dogs, cats, cattle, and pigs sampled before 2019 (n = 170), considering a 98% percentile and a grey zone to completely exclude any false positive result. Specificity was confirmed by measuring levels of neutralizing antibodies against canine coronavirus, the avidity of specific antibodies, and their capacity to impede the binding of a recombinant RBD protein to VERO cells in an In-Cell ELISA. Sera from 464 cats and dogs sampled in 2020 and 2021 (“pandemic” samples) were assessed using the RBD-ELISA. Information on COVID-19 disease in the household and the animals’ lifestyles was collected. In Buenos Aires Suburbs cats were infected at a higher proportion than dogs, seroprevalence was 7.1 and 1.68%, respectively. Confirmed COVID-19 in the caregivers and outdoor lifestyle were statistically associated with seropositivity in cats. The risk of cats getting infected living indoors in COVID-19-negative households was null. The susceptibility of mammals to SARS-CoV-2, the possibility of transmission between animals themselves and humans, together with the free-roaming lifestyle typical of Buenos Aires suburban companion animals, urge pursuing responsible animal care and avoiding human interaction with animals during the disease course. The multi-species RBD-ELISA we developed can be used as a tool for serosurveillance of SARS-CoV-2 infection in mammalians (domestic and wild), guiding further targeted virological analyses to encounter susceptible species, interspecies transmission, and potential virus reservoirs in our region. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Frontiers Media | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud? | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | es_AR |
dc.source | Frontiers in Veterinary Science 10 : 1161820. (May 2023) | es_AR |
dc.subject | COVID-19 | eng |
dc.subject | Coronavirus del Síndrome Respiratorio Agudo Grave 2 | es_AR |
dc.subject | Severe Acute Respiratory Syndrome Coronavirus 2 | eng |
dc.subject | Animales Domésticos | es_AR |
dc.subject | Domestic Animals | eng |
dc.subject | Inmunodiagnóstico | es_AR |
dc.subject | Immunodiagnosis | eng |
dc.subject | ELISA | eng |
dc.subject | Evaluación Preliminar | es_AR |
dc.subject | Screening | eng |
dc.subject.other | SARS-CoV-2 | es_AR |
dc.subject.other | Suburbios de Buenos Aires | es_AR |
dc.title | Serological screening of SARS-CoV-2 infection in companion animals of Buenos Aires suburbs | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | es_AR |
dc.description.origen | Instituto de Virología | es_AR |
dc.description.fil | Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | es_AR |
dc.description.fil | Fil: Cardoso, Nancy Patricia. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina | es_AR |
dc.description.fil | Fil: Rivero, Carla. Universidad Nacional de José Clemente Paz. Instituto de Estudios para el Desarrollo Productivo y la Innovación; Argentina | es_AR |
dc.description.fil | Fil: Castillo, Mariangeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | es_AR |
dc.description.fil | Fil: Castillo, Mariangeles. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina | es_AR |
dc.description.fil | Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. | es_AR |
dc.description.fil | Fil: Pastorino, Florencia. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina | es_AR |
dc.description.fil | Fil: Piccirilli, Guadalupe. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina | es_AR |
dc.description.fil | Fil: Alonso, Maria Laura. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina | es_AR |
dc.description.fil | Fil: Martinez, Gustavo. Provincia de Buenos Aires. Ministerio de Salud. Departamento Zoonosis Urbanas; Argentina | es_AR |
dc.description.fil | Fil: Di Lullo, David. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo; Argentina | es_AR |
dc.description.fil | Fil: Di Lullo, David. Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo; Argentina | es_AR |
dc.description.fil | Fil: Bentancor, Leticia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Bentancor, Leticia Veronica. Universidad Nacional de José Clemente Paz. Instituto de Estudios para el Desarrollo Productivo y la Innovación; Argentina | es_AR |
dc.description.fil | Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | es_AR |
dc.description.fil | Fil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
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