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Resumen
Profilins are actin-binding proteins that regulate the polymerization of actin filaments. In apicomplexan parasites, they are essential for invasion. Profilins also trigger the immune response of the host by activating TLRs on dendritic cells (DCs), inducing the production of pro-inflammatory cytokines. In this study we characterized for the first time the immune response and protection elicited by a vaccine based on Neospora caninum profilin in mice. [ver mas...]
dc.contributor.authorMansilla, Florencia Celeste
dc.contributor.authorQuintana, Maria Eugenia
dc.contributor.authorLangellotti, Cecilia Ana
dc.contributor.authorWilda, Maximiliano
dc.contributor.authorMartinez, Andrea
dc.contributor.authorFonzo, Adriana
dc.contributor.authorMoore, Prando Dadin
dc.contributor.authorCardoso, Nancy
dc.contributor.authorCapozzo, Alejandra
dc.date.accessioned2017-10-02T12:25:42Z
dc.date.available2017-10-02T12:25:42Z
dc.date.issued2016
dc.identifier.issn0014-4894 (Print)
dc.identifier.issn1090-2449 (Online)
dc.identifier.otherhttps://doi.org/10.1016/j.exppara.2015.10.008
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1370
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0014489415300552
dc.description.abstractProfilins are actin-binding proteins that regulate the polymerization of actin filaments. In apicomplexan parasites, they are essential for invasion. Profilins also trigger the immune response of the host by activating TLRs on dendritic cells (DCs), inducing the production of pro-inflammatory cytokines. In this study we characterized for the first time the immune response and protection elicited by a vaccine based on Neospora caninum profilin in mice. Groups of eight BALB/c mice received either two doses of a recombinant N. caninum profilin expressed in Escherichia coli. (rNcPRO) or PBS, both formulated with an aqueous soy-based adjuvant enriched in TLR-agonists. Specific anti-profilin antibodies were detected in rNcPRO-vaccinated animals, mainly IgM and IgG3, which were consumed after infection. Splenocytes from rNcPRO-immunized animals proliferated after an in vitro stimulation with rNcPRO before and after challenge. An impairment of the cellular response was observed in NcPRO vaccinated and infected mice following an in vitro stimulation with native antigens of N. caninum, related to an increase in the percentage of CD4þCD25þFoxP3þ. Two out of five rNcPRO-vaccinated challenged mice were protected; they were negative for parasite DNA in the brain and showed no histopathological lesions, which were found in all PBS-vaccinated animals. As a whole, our results provide evidence of a regulatory response elicited by immunization with rNcPRO, and suggest a role of profilin in the modulation and/or evasion of immune responses against N. caninum.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceExperimental parasitology 160 : 1-10. (January 2016)
dc.subjectEnfermedades de los Animales
dc.subjectAnimal Diseaseseng
dc.subjectNeospora Caninum
dc.subjectInmunización
dc.subjectImmunizationeng
dc.subjectLinfocitos-t
dc.subjectT-lymphocyteseng
dc.subjectRatón
dc.subjectMiceeng
dc.titleImmunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in miceeng
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/acceptedVersioneng
dc.description.origenInst.de Virología
dc.gic617
dc.description.filFil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Quintana, Maria Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Wilda, Maximiliano. Tecnovax S.A,; Argentina
dc.description.filFil: Martinez, Andrea. Instituto de Ciencia y Tecnología Dr. Céesar Milstein; Argentina
dc.description.filFil: Fonzo, Adriana. Instituto de Ciencia y Tecnología Dr. Céesar Milstein; Argentina
dc.description.filFil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
dc.description.filFil: Cardoso, Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.subtypecientifico


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