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Abstract
Clostridioides difficile (C. difficile) is a gram-positive, spore-forming, anaerobic bacterium known to be the most common cause of hospital-acquired and antibiotic-associated diarrhea. C. difficile infection rates are on the rise worldwide and treatment options are limited, indicating a clear need for novel therapeutics. Gnotobiotic piglets are an excellent model to reproduce the acute pseudomembranous colitis (PMC) caused by C. difficile due to their [ver mas...]
dc.contributor.authorNyblade, Charlotte
dc.contributor.authorParreño, Gladys Viviana
dc.contributor.authorZhou, Peng
dc.contributor.authorHensley, Casey
dc.contributor.authorOakes, Vanessa
dc.contributor.authorMahsoub, Hassan M.
dc.contributor.authorKiley, Kelsey
dc.contributor.authorFrazier, Maggie
dc.contributor.authorFrazier, Annie
dc.contributor.authorZhang, Yongrong
dc.contributor.authorFeng, Hanping
dc.contributor.authorYuan, Lijuan
dc.date.accessioned2022-07-19T16:32:40Z
dc.date.available2022-07-19T16:32:40Z
dc.date.issued2022-06
dc.identifier.issn1757-4749
dc.identifier.otherhttps://doi.org/10.1186/s13099-022-00496-y
dc.identifier.urihttp://hdl.handle.net/20.500.12123/12350
dc.identifier.urihttps://gutpathogens.biomedcentral.com/articles/10.1186/s13099-022-00496-y
dc.description.abstractClostridioides difficile (C. difficile) is a gram-positive, spore-forming, anaerobic bacterium known to be the most common cause of hospital-acquired and antibiotic-associated diarrhea. C. difficile infection rates are on the rise worldwide and treatment options are limited, indicating a clear need for novel therapeutics. Gnotobiotic piglets are an excellent model to reproduce the acute pseudomembranous colitis (PMC) caused by C. difficile due to their physiological similarities to humans and high susceptibility to infection. Here, we established a gnotobiotic pig model of C. difficile infection and disease using a hypervirulent strain. C. difficile-infected pigs displayed classic signs of C. difficile infection, including severe diarrhea and weight loss. Inoculated pigs had severe gross and microscopic intestinal lesions. C. difficile infection caused an increase in pro-inflammatory cytokines in samples of serum, large intestinal contents, and pleural effusion. C. difficile spores and toxins were detected in the feces of inoculated animals as tested by anaerobic culture and cytotoxicity assays. Successful establishment of this model is key for future work as therapeutics can be evaluated in an environment that accurately mimics what happens in humans. The model is especially suitable for evaluating potential prophylactics and therapeutics, including vaccines and passive immune strategies.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherBioMed Centrales_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceGut Pathogens 14 : 22 (Junio 2022)es_AR
dc.subjectGnotobiotic Animalseng
dc.subjectAnimales Notobióticoses_AR
dc.subjectSwineeng
dc.subjectCerdoes_AR
dc.subject.otherClostridioides difficilees_AR
dc.subject.otherPseudomembranous colitises_AR
dc.subject.otherColitis pseudomembranosaes_AR
dc.titleEstablishment of a gnotobiotic pig model of Clostridioides difficile infection and diseasees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Virologíaes_AR
dc.description.filFil: Nyblade, Charlotte. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidoses_AR
dc.description.filFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.description.filFil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Parreño, Gladys Viviana. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidoses_AR
dc.description.filFil: Zhou, Peng. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidoses_AR
dc.description.filFil: Hensley, Casey. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidoses_AR
dc.description.filFil: Oakes, Vanessa. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidoses_AR
dc.description.filFil: Mahsoub, Hassan M. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidoses_AR
dc.description.filFil: Mahsoub, Hassan M. Virginia Polytechnic Institute and State University. Center for Emerging, Zoonotic, and Arthropod‑Borne Pathogens; Estados Unidoses_AR
dc.description.filFil: Kiley, Kelsey. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidoses_AR
dc.description.filFil: Frazier, Maggie. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidoses_AR
dc.description.filFil: Frazier, Annie. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidoses_AR
dc.description.filFil: Zhang, Yongrong. University of Maryland at Baltimore. Department of Microbial Pathogenesis; Estados Unidoses_AR
dc.description.filFil: Feng, Hanping. University of Maryland at Baltimore. Department of Microbial Pathogenesis; Estados Unidoses_AR
dc.description.filFil: Yuan, Lijuan. Virginia Polytechnic Institute and State University. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidoses_AR
dc.description.filFil: Yuan, Lijuan. Virginia Polytechnic Institute and State University. Center for Emerging, Zoonotic, and Arthropod‑Borne Pathogens; Estados Unidoses_AR
dc.subtypecientifico


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