Mostrar el registro sencillo del ítem
resumen
Resumen
Infectious bronchitis virus (Gammacoronavirus, Coronaviridae) is a genetically variable RNA virus (27.6kb) that causes one of the most persistent respiratory disease in poultry. The virus is classified in genotypes with different epidemiological relevance and clinical implications. The present study reports the development and validation of specific RT-qPCR assays for the detection of two major IBV genotypes: South America I (SAI) and Asia/South America
[ver mas...]
dc.contributor.author | Marandino, Ana | |
dc.contributor.author | Tomás, Gonzalo | |
dc.contributor.author | Hernández, Martín | |
dc.contributor.author | Panzera, Yanina | |
dc.contributor.author | Craig, María Isabel | |
dc.contributor.author | Vagnozzi, Ariel Eduardo | |
dc.contributor.author | Vera, Federico Sebastian | |
dc.contributor.author | Techera, Claudia | |
dc.contributor.author | Grecco, Sofía | |
dc.contributor.author | Banda, Alejandro | |
dc.contributor.author | Hernández, Diego | |
dc.contributor.author | Pérez, Ruben | |
dc.date.accessioned | 2017-09-14T17:08:05Z | |
dc.date.available | 2017-09-14T17:08:05Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0166-0934 (Print) | |
dc.identifier.issn | 1879-0984 (Online) | |
dc.identifier.other | https://doi.org/10.1016/j.jviromet.2016.05.007 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/1224 | |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0166093416300325 | |
dc.description.abstract | Infectious bronchitis virus (Gammacoronavirus, Coronaviridae) is a genetically variable RNA virus (27.6kb) that causes one of the most persistent respiratory disease in poultry. The virus is classified in genotypes with different epidemiological relevance and clinical implications. The present study reports the development and validation of specific RT-qPCR assays for the detection of two major IBV genotypes: South America I (SAI) and Asia/South America II (A/SAII). The SAI genotype is an exclusive and widespread South American lineage while the A/SAII genotype is distributed in Asia, Europe and South America. Both identification assays employ TaqMan probes that hybridize with unique sequences in the spike glycoprotein gene. The assays successfully detected all the assessed strains belonging to both genotypes, showing high specificity and absence of cross-reactivity. Using serial dilutions of in vitro-transcribed RNA we obtained acceptable determination coefficients, PCR efficiencies and relatively small intra- and inter-assay variability. The assays demonstrated a wide dynamic range between 10(1)-10(7) and 10(2)-10(7) RNA copies/reaction for SAI and A/SAII strains, respectively. The possibility to characterize a large number of samples in a rapid, sensitive and reproducible way makes these techniques suitable tools for routine testing, IBV control, and epidemiological research in poultry | eng |
dc.format | application/pdf | |
dc.language.iso | eng | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.source | Journal of virological methods 235 : 21-25. (September 2016) | |
dc.subject | Enfermedades de los Animales | |
dc.subject | Animal Diseases | eng |
dc.subject | Virus Bronquitis Infecciosa Aviar | |
dc.subject | Avian Infectious Bronchitis Virus | eng |
dc.subject | PCR | eng |
dc.subject | Genotipos | |
dc.subject | Genotypes | eng |
dc.subject | Aves de Corral | |
dc.subject | Poultry | eng |
dc.title | Development of RT-qPCR assays for the specific identification of two major genotypes of avian infectious bronchitis virus | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/acceptedVersion | |
dc.description.origen | Inst.de Virología | |
dc.gic | 150802 | |
dc.description.fil | Fil: Marandino, Ana. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva; Uruguay | |
dc.description.fil | Fil: Tomás, Gonzalo. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva; Uruguay | |
dc.description.fil | Fil: Hernández, Martín. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva; Uruguay | |
dc.description.fil | Fil: Panzera, Yanina. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva; Uruguay | |
dc.description.fil | Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | |
dc.description.fil | Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | |
dc.description.fil | Fil: Vera, Federico Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina | |
dc.description.fil | Fil: Techera, Claudia. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva; Uruguay | |
dc.description.fil | Fil: Grecco, Sofía. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva; Uruguay | |
dc.description.fil | Fil: Banda, Alejandro. Mississippi State University. College of Veterinary Medicine. Poultry Research and Diagnostic Laboratory; Estados Unidos | |
dc.description.fil | Fil: Hernández, Diego. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva; Uruguay | |
dc.description.fil | Fil: Pérez, Ruben. Universidad de la República. Facultad de Ciencias. Instituto de Biología. Sección Genética Evolutiva; Uruguay | |
dc.subtype | cientifico |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
common
-
Artículos científicos [197]