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Background: In 2012, equine influenza (EI) virus was confirmed as the cause of outbreaks of respiratory disease in horses throughout South America. In Uruguay and Argentina, hundreds of vaccinated thoroughbred horses in training and racing facilities were clinically affected. Objective: To characterise the EI viruses detected during the outbreak in Uruguay and Argentina. Methods: Virus was detected in nasopharyngeal swabs by a pan-reactive influenza [ver mas...]
dc.contributor.authorOlguin Perglione, Cecilia
dc.contributor.authorRimondi, Agustina
dc.contributor.authorGildea, Sarah
dc.contributor.authorMiño, Samuel
dc.contributor.authorVissani, Aldana
dc.contributor.authorCarossino, Mariano
dc.contributor.authorCullinane, Ann
dc.contributor.authorBarrandeguy, Maria Edith
dc.coverage.temporal2012
dc.date.accessioned2017-09-14T14:53:57Z
dc.date.available2017-09-14T14:53:57Z
dc.date.issued2016-01
dc.identifier.issn1750-2659
dc.identifier.otherDOI: 10.1111/irv.12349
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1222
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1111/irv.12349/epdf
dc.description.abstractBackground: In 2012, equine influenza (EI) virus was confirmed as the cause of outbreaks of respiratory disease in horses throughout South America. In Uruguay and Argentina, hundreds of vaccinated thoroughbred horses in training and racing facilities were clinically affected. Objective: To characterise the EI viruses detected during the outbreak in Uruguay and Argentina. Methods: Virus was detected in nasopharyngeal swabs by a pan-reactive influenza type A real-time RT-PCR. The nucleotide sequence of the HA1 gene was determined and analysed phylogenetically using mega 5 software. Amino acid sequences alignments were constructed and virus was antigenically characterised with specific ferret antisera. Paired serum samples were tested by haemagglutination inhibition and single radial haemolysis. Results: The diagnosis of EIV was confirmed by real-time RT-PCR, virus isolation and serological testing. The phylogenetic analysis of HA1 gene sequences of 18 EI viruses indicated that all of them belong to clade 1 of the Florida sublineage of the American lineage and are closely related to viruses isolated in the United States in 2012. The HA1 of viruses identified in horses in racing facilities in Maroñas, Uruguay, and in Palermo, Argentina, displayed 100% amino acid sequence identity and were identical to that of a virus isolated in Dubai in 2012, from vaccinated endurance horses recently imported from Uruguay. Conclusions: The surveillance data reported illustrate the international spread of EI viruses and support the recommendations of the OIE expert surveillance panel to include viruses of the Florida sublineage in vaccines.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceInfluenza and Other Respiratory Viruses 10 (1) : 37–46 (January 2016)
dc.subjectEquine Influenzaviruseng
dc.subjectVirus de la Influenza Equinaes_AR
dc.subjectEnfermedades de los Animales
dc.subjectAnimal Diseaseseng
dc.subjectCaballos
dc.subjectHorseseng
dc.subjectEpidemiología
dc.subjectEpidemiologyeng
dc.subject.otherAmérica del Sur
dc.titleEpidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012es_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.filFil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Gildea, Sarah. The Irish Equine Centre. Virology Unit; Irlanda
dc.description.filFil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Vissani, Aldana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Carossino, Mariano. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos
dc.description.filFil: Cullinane, Ann. The Irish Equine Centre. Virology Unit; Irlanda
dc.description.filFil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria. Cátedra de Enfermedades Infecciosas; Argentina
dc.subtypecientifico


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