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Abstract
This study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro -grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limo-nene, 1,8-cineole and c-terpinene, [ver mas...]
dc.contributor.authorPontin, Mariela Ana
dc.contributor.authorPiccoli, Patricia Noemí
dc.contributor.authorBurba, Jose Luis
dc.contributor.authorBottini, Ambrosio Rubén
dc.coverage.spatialMendoza (province)eng
dc.date.accessioned2017-09-07T12:46:16Z
dc.date.available2017-09-07T12:46:16Z
dc.date.issued2015-07
dc.identifier.issn0031-9422 (Print)
dc.identifier.issn1873-3700 (Online)
dc.identifier.otherhttps://doi.org/10.1016/j.phytochem.2015.02.003
dc.identifier.urihttp://hdl.handle.net/20.500.12123/1161
dc.identifier.urihttp://ri.conicet.gov.ar/handle/11336/22405
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0031942215000503?via%3Dihub
dc.description.abstractThis study investigated terpene biosynthesis in different tissues (root, protobulb, leaf sheath and blade) of in vitro -grown garlic plants either infected or not (control) with Sclerotium cepivorum, the causative agent of Allium White Rot disease. The terpenes identified by gas chromatography–electron impact mass spectrometry (GC-EIMS) in infected plants were nerolidol, phytol, squalene, a-pinene, terpinolene, limo-nene, 1,8-cineole and c-terpinene, whose levels significantly increased when exposed to the fungus. Consistent with this, an increase in terpene synthase (TPS) activity was measured in infected plants. Among the terpenes identified, nerolidol, a-pinene and terpinolene were the most abundant with antifungal activity against S. cepivorum being assessed in vitro by mycelium growth inhibition. Nerolidol and terpinolene significantly reduced sclerotia production, while a-pinene stimulated it in a concentration-dependent manner. Parallel to fungal growth inhibition, electron microscopy observations established morphological alterations in the hyphae exposed to terpinolene and nerolidol. Differences in hyphal EtBr uptake suggested that one of the antifungal mechanisms of nerolidol and terpinolene migh be disruption of fungal membrane integrityeng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourcePhytochemistry 115 :152-160. (July 2015)eng
dc.subjectEcología de la planta
dc.subjectAllium Sativum
dc.subjectAjo
dc.subjectGarliceng
dc.subjectTerpenoidos
dc.subjectTerpenoidseng
dc.subjectSclerotium Cepivorum
dc.subjectPropiedades Antimicósicas
dc.subjectAntifungal Propertieseng
dc.subject.otherTerpenos
dc.titleAllium sativum produces terpenes with fungistatic properties in response to infection with Sclerotium cepivorumeng
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/acceptedVersioneng
dc.description.origenEEA La Consulta
dc.gic962
dc.description.filFil: Pontin, Mariela Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
dc.description.filFil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
dc.description.filFil: Burba, José Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
dc.description.filFil: Bottini, Ambrosio Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
dc.subtypecientifico


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