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Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species
Resumen
Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus.
Area of the study: samples were obtained from natural populations of Argentina.
Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and
[ver mas...]
Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus.
Area of the study: samples were obtained from natural populations of Argentina.
Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and interspecific hybrids.
Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci.
Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers.
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Fuente
Forest Systems 24 (2) : eRC04 (2015)
Fecha
2015
Editorial
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
ISSN
2171-9845
2171-5068
2171-5068
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pdf
Tipo de documento
artículo
Proyectos
(ver más)
INTA/PNBIO-1131044/AR./Genómica aplicada a estudios de ecología molecular y diversidad genética.
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