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Absence of the nitrous oxide reductase gene cluster in commercial alfalfa inoculants is probably due to the extensive loss of genes during rhizobial domestication
Brambilla, Silvina Maricel; Frare, Romina Alejandra; Soto, Gabriela Cinthia; Jozefkowicz, Cintia; Ayub, Nicolás Daniel (Springer, 2018-08)As other legume crops, alfalfa cultivation increases the emission of the greenhouse gas nitrous oxide (N2O). Since legumesymbiotic nitrogen-fixing bacteria play a crucial role in this emission, it is important to understand ... -
Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
Garcia, Araceli Nora; Ayub, Nicolás Daniel; Fox, Ana Romina; Gomez, Maria Cristina; Dieguez, Maria Jose; Pagano, Elba Maria; Berini, Carolina Andrea; Muschietti, Jorge Prometeo; Soto, Gabriela Cinthia (BMC Plant Biology, 2014-09-17)Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. ... -
Cooperativity in proton sensing by PIP aquaporins
Vitali, Victoria Andrea; Jozefkowicz, Cintia; Canessa Fortuna, Agustina; Soto, Gabriela Cinthia; Gonzalez Flecha, Francisco Luis; Alleva, Karina Edith (Wiley, 2019-03)One of the most intriguing properties of plasma membrane intrinsic protein (PIP) aquaporins (AQPs) is their ability to modulate water transport by sensing different levels of intracellular pH through the assembly of homo‐ ... -
Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions
Setten, Lorena; Soto, Gabriela Cinthia; Mozzicafreddo, Matteo; Fox, Ana Romina; Lisi, Christian Daniel; Cuccioloni, Massimiliano; Angeletti, Mauro; Pagano, Elba Maria; Diaz Paleo, Antonio Horacio; Ayub, Nicolás Daniel (2013-05-13)Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the ... -
Expanding the benefits of Tnt1 for the identification of dominant mutations in polyploid crops: A single allelic mutation in the MsNAC39 gene produces multifoliated alfalfa
Jozefkowicz, Cintia; Gomez, Maria Cristina; Odorizzi, Ariel; Iantcheva, Anelia; Ratet, Pascal; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Frontiers Media, 2022-04)Most major crops are polyploid species and the production of genetically engineered cultivars normally requires the introgression of transgenic or gene-edited traits into elite germplasm. Thus, a main goal of plant research ... -
Exploring the ancestral mechanisms of regulation of horizontally acquired nitrogenases
Pascuan, Cecilia Gabriela; Fox, Ana Romina; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (Springer, 2015-10)The vast majority of Pseudomonas species are unable to fix atmospheric nitrogen. Although several studies have demonstrated that some strains belonging to the genus Pseudomonas sensu stricto do have the ability to fix ... -
Exploring the role of the NO‑detoxifying enzyme HmpA in the evolution of domesticated Alfalfa Rhizobia
Frare, Romina Alejandra; Pascuan, Cecilia Gabriela; Galindo Sotomonte, Luisa Fernanda; McCormick, Wayne; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2022-02)We have previously shown the extensive loss of genes during the domestication of alfalfa rhizobia and the high nitrous oxide emission associated with the extreme genomic instability of commercial inoculants. In the present ... -
Expression analysis of genes putatively associated with hygienic behavior in selected stocks of Apis mellifera L. from Argentina
Scannapieco, Alejandra Carla; Mannino, Constanza; Soto, Gabriela Cinthia; Palacio, María Alejandra; Cladera, Jorge Luis; Lanzavecchia, Silvia Beatriz (2017-11)Hygienic behavior is an economically beneficial, heritable trait, which has evolved to limit the impact of honeybee pathogens. Selecting and breeding colonies with high levels of hygienic behavior has become a feasible and ... -
Generation of a multi‑herbicide‑tolerant alfalfa by using base editing
Bottero, Ana Emilia; Gomez, Maria Cristina; Stritzler, Margarita; Tajima, Hiromi; Frare, Romina Alejandra; Pascuan, Cecilia Gabriela; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2022-02)We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important ... -
Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island
Iribarren, Maria Josefina; Pascuan, Cecilia Gabriela; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Oxford University Press, 2015-11)Phytophthora capsici is a virulent oomycete pathogen of many vegetable crops. Recently, it has been demonstrated that the recognition of the RXLR effector AVR3a1 of P. capsici (PcAVR3a1) triggers a hypersensitive response ... -
High-quality forage production under salinity by using a salt-tolerant AtNXH1-expressing transgenic alfalfa combined with a natural stress-resistant nitrogen-fixing bacterium
Stritzler, Margarita; Pagano, Elba Maria; Berini, Carolina Andrea; Gomez, Maria Cristina; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia (Elsevier, 2018-06)Alfalfa, usually known as the “Queen of Forages”, is the main source of vegetable protein to meat and milk production systems worldwide. This legume is extremely rich in proteins due to its highly efficient symbiotic ... -
Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940
Fox, Ana Romina; Soto, Gabriela Cinthia; Valverde, Claudio Fabián; Russo, Daniela Marta; Lagares, Antonio; Zorreguieta, Ángeles; Pascuan, Cecilia Gabriela; Frare, Romina Alejandra; Mercado Blanco, Jesús; Dixon, Ray; Ayub, Nicolás Daniel; Alleva, Karina Edith (2016)A main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability to major cereal crops. In this work, we demonstrate the use of the efficient nitrogen-fixing rhizobacterium Pseudomonas ... -
Maximizing the expression of transgenic traits into elite alfalfa germplasm using a supertransgene configuration in heterozygous conditions
Jozefkowicz, Cintia; Frare, Romina Alejandra; Odorizzi, Ariel; Arolfo, Valeria; Pagano, Elba Maria; Basigalup, Daniel Horacio; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia (2018-05)Numerous species of legumes, including alfalfa, are critical factors for agroecosystems due to their ability to grow without nitrogen fertilizers derived from non-renewable fossil fuels, their contribution of organic ... -
Microevolution rather than large genome divergence determines the effectiveness of legume–rhizobia symbiotic interaction under field conditions
Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Puente, Mariana Laura; Piccinetti, Carlos Fabian; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (2017-08)Despite the vast screening for natural nitrogenfixing isolates by public and private consortia, no significant progresses in the production of improved nitrogenfixing inoculants for alfalfa production have been made in the ... -
Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR
Bottero, Ana Emilia; Pascuan, Cecilia Gabriela; Pagano, Elba Maria; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia; Jozefkowicz, Cintia (2016)Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the ... -
mRNA biogenesis-related helicase eIF4AIII from Arabidopsis thaliana is an important factor for abiotic stress adaptation
Pascuan, Cecilia Gabriela; Frare, Romina Alejandra; Alleva, Karina Edith; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia (2016)Similar to other plant species, Arabidopsis has a huge repertoire of predicted helicases, including the eIF4AIII factor, a putative component of the exon junction complex related to mRNA biogenesis. In this article, we ... -
pBAR–H3.2, a native-optimized binary vector to bypass transgene silencing in alfalfa
Pascuan, Cecilia Gabriela; Bottero, Ana Emilia; Kapros, Tamas; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2020-03)Alfalfa is the main forage worldwide due to its high biomass production, excellent nutritional qualities and adaptation to a wide range of environments (Singer et al. 2018). Besides, due to its ability to grow without ... -
PIP aquaporin pH sensing is regulated by the length and charge of the C-terminal region
Scochera, Florencia; Zerbetto De Palma, Gerardo; Canessa Fortuna, Agustina; Chevriau, Jonathan; Toriano, Roxana; Soto, Gabriela Cynthia; Zeida, Ari; Alleva, Karina Edith (Wiley, 2021-07)Plant PIP aquaporins play a central role in controlling plant water status. The current structural model for PIP pH-gating states that the main pH sensor is located in loopD and that all the mobile cytosolic elements ... -
Pip water transport and its pH dependence are regulated by tetramer stoichiometry
Jozefkowicz, Cintia; Sigaut, Lorena; Scochera, Florencia; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel; Pietrasanta, Lía Isabel; Amodeo, Gabriela; Gonzalez Flecha, Francisco Luis; Alleva, Karina Edith (2016)Many plasma membrane channels form oligomeric assemblies, and heterooligomerization has been described as a distinctive feature of some protein families. In the particular case of plant plasma membrane aquaporins (PIPs), ... -
Pollen aquaporins : the solute factor
Perez Di Giorgio, Juliana Andrea; Soto, Gabriela Cinthia; Muschietti, Jorge Prometeo; Amodeo, Gabriela (2016)In the recent years, the biophysical properties and presumed physiological role of aquaporins (AQPs) have been expanded to specialized cells where water and solute exchange are crucial traits. Complex but unique processes ...