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Multiple challenges in the development of commercial crops using CRISPR technology
Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Elsevier, 2023-10)The CRISPR/Cas system is a highly efficient and versatile tool for editing plant genomes, with the potential to accelerate breeding programs and improve the sustainability of food production. Nevertheless, technical ... -
Multiple ways to evade the bacteriostatic action of glyphosate in rhizobia include the mutation of the conserved serine 90 of the nitrogenase subunit NifH to alanine
Liebrenz, Karen Ivana; Frare, Romina Alejandra; Gomez, Maria Cristina; Pascuan, Cecilia Gabriela; Brambilla, Silvina Maricel; Soldini, Diego Omar; Maguire, Vanina; Carrio, Alejandro Javier; Ruiz, Oscar; McCormick, Wayne; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Elsevier, 2022-07)The genome resequencing of spontaneous glyphosate-resistant mutants derived from the soybean inoculant E109 allowed identifying genes most likely associated with the uptake (gltL and cya) and metabolism (zigA and betA) of ... -
pBAR–H3.2, a native-optimized binary vector to bypass transgene silencing in alfalfa
Pascuan, Cecilia Gabriela; Bottero, Ana Emilia; Kapros, Tamas; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2020-03)Alfalfa is the main forage worldwide due to its high biomass production, excellent nutritional qualities and adaptation to a wide range of environments (Singer et al. 2018). Besides, due to its ability to grow without ... -
PIP aquaporin pH sensing is regulated by the length and charge of the C-terminal region
Scochera, Florencia; Zerbetto De Palma, Gerardo; Canessa Fortuna, Agustina; Chevriau, Jonathan; Toriano, Roxana; Soto, Gabriela Cynthia; Zeida, Ari; Alleva, Karina Edith (Wiley, 2021-07)Plant PIP aquaporins play a central role in controlling plant water status. The current structural model for PIP pH-gating states that the main pH sensor is located in loopD and that all the mobile cytosolic elements ... -
Pip water transport and its pH dependence are regulated by tetramer stoichiometry
Jozefkowicz, Cintia; Sigaut, Lorena; Scochera, Florencia; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel; Pietrasanta, Lía Isabel; Amodeo, Gabriela; Gonzalez Flecha, Francisco Luis; Alleva, Karina Edith (2016)Many plasma membrane channels form oligomeric assemblies, and heterooligomerization has been described as a distinctive feature of some protein families. In the particular case of plant plasma membrane aquaporins (PIPs), ... -
Pollen aquaporins : the solute factor
Perez Di Giorgio, Juliana Andrea; Soto, Gabriela Cinthia; Muschietti, Jorge Prometeo; Amodeo, Gabriela (2016)In the recent years, the biophysical properties and presumed physiological role of aquaporins (AQPs) have been expanded to specialized cells where water and solute exchange are crucial traits. Complex but unique processes ... -
Pollen-specific aquaporins NIP4;1 and NIP4;2 are required for pollen development and pollination in Arabidopsis thaliana
Perez Di Giorgio, Juliana Andrea; Bienert, Gerd Patrick; Ayub, Nicolás Daniel; Yaneff, Agustín; Barberini, María Laura; Mecchia, Martín Alejandro; Amodeo, Gabriela; Soto, Gabriela Cinthia; Muschietti, Jorge Prometeo (2016)In flowers with dry stigmas, pollen development, pollination, and pollen tube growth require spatial and temporal regulation of water and nutrient transport. To better understand the molecular mechanisms involved in ... -
Prediction of aquaporin function by integrating evolutionary and functional analyses
Perez Di Giorgio, Juliana Andrea; Soto, Gabriela Cinthia; Alleva, Karina Edith; Jozefkowicz, Cintia; Amodeo, Gabriela; Muschietti, Jorge Prometeo; Ayub, Nicolás Daniel (Springer, 2014-02)Aquaporins (AQPs) are a family of channel proteins, which transport water and/or small solutes across cell membranes. AQPs are present in Bacteria, Eukarya, and Archaea. The classical AQP evolution paradigm explains the ... -
Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
Ayub, Nicolás Daniel; Fox, Ana Romina; Garcia, Araceli Nora; Mozzicafreddo, Matteo; Cuccioloni, Massimiliano; Angeletti, Mauro; Pagano, Elba Maria; Soto, Gabriela Cynthia (Oxford University Press, 2015-01)Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. ... -
Rapid and cloning‑free screening of edited alfalfa via next‑generation sequencing
Stritzler, Margarita; Pascuan, Cecilia Gabriela; Bottero, Ana Emilia; Gomez, Maria Cristina; Frare, Romina Alejandra; Puebla, Andrea Fabiana; Tajima, Hiromi; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)The identification of full knockout plants is frequently the rate-limiting step of the research and commercial projects searching the complete loss of the function of target genes via the CRISPR/Cas9 system. This problem ... -
Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
Frare, Romina Alejandra; Stritzler, Margarita; Gomez, Maria Cristina; Tajima, Hiromi; Pascuan, Cecilia Gabriela; López Fernández, María Paula; Bottero, Ana Emilia; Nikel, Pablo I.; Alleva, Karina Edith; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities ... -
Root hydraulic conductivity and adjustments in stomatal conductance: hydraulic strategy in response to salt stress in a halotolerant species
Vitali, Victoria Andrea; Bellati, Jorge; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel; Amodeo, Gabriela (Oxford University Press, 2015-11)Recent advances at the molecular level are introducing a new scenario that needs to be integrated into the analysis of plant hydraulic properties. Although it is not yet clear to what extent this scenario alters the current ... -
Stable symbiotic nitrogen fixation under water-deficit field conditions by a stress-tolerant alfalfa microsymbiont and its complete genome sequence : Short communication
Jozefkowicz, Cintia; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Piccinetti, Carlos Fabian; Puente, Mariana Laura; Berini, Carolina Andrea; Reyes Perez, Pedro Jose; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (2017-12)We here characterized the stress-tolerant alfalfa microsymbiont Sinorhizobium meliloti B401. B401-treated plants showed high nitrogen fixation rates under humid and semiarid environments. The production of glycine betaine ... -
Subfunctionalization probably drives the emergence of plant growth‑promoting genes
Frare, Romina Alejandra; Mozzicafreddo, Matteo; Gomez, Maria Cristina; Fussoni, Nerina Belen; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2022-07)The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution ... -
Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils
Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Soto, Gabriela Cynthia; Berini, Carolina Andrea; Jozefkowicz, Cintia; Ayub, Nicolás Daniel (Elsevier, 2020-09)Objectives: Unlike higher organisms such as domestic animals and cultivated plants, which display a robust reproductive isolation and limited dispersal ability, microbes exhibit an extremely promiscuous gene flow and can ... -
The ammonium channel NOD26 is the evolutionary innovation that drives the emergence, consolidation, and dissemination of nitrogen-fixing symbiosis in angiosperms
Frare, Romina Alejandra; Ayub, Nicolás Daniel; Alleva, Karina Edith; Soto, Gabriela Cinthia (Springer, 2018-10)Increasing evidence indicates that N-fixing symbiosis has evolved several times in the N-fixing clade of angiosperms and that this evolution is driven by a single evolutionary innovation. However, the genetics of this ... -
The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa
Gomez, Maria Cristina; Jozefkowicz, Cintia; Mozzicafreddo, Matteo; Odorizzi, Ariel; Arolfo, Valeria; Basigalup, Daniel Horacio; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2022-12)The CRISPR/Cas9 system has proven to be highly valuable for genome editing in several important crops, including the allogamous tetraploid cultivated alfalfa (Medicago sativa). However, the fact that most of the beneficial ... -
Understanding the intracellular-to-extracellular localization switch of polyhydroxybutyrate polymerase in pseudomonas backgrounds as a microevolutionary process
Stritzler, Margarita; Berini, Carolina Andrea; Jozefkowicz, Cintia; Soto, Gabriela Cinthia; Ayub, Nicolás Daniel (Elsevier, 2018-11)After gene duplication, paralogous genes evolve independently, and consequently, the new proteins encoded by these duplicated genes are exposed to changes in their subcellular location. Although there are increasing evidence ... -
Whole-Genome Resequencing of Spontaneous Oxidative Stress-Resistant Mutants Reveals an Antioxidant System of Bradyrhizobium japonicum Involved in Soybean Colonization
Liebrenz, Karen Ivana; Gomez, Maria Cristina; Brambilla, Silvina Maricel; Frare, Romina Alejandra; Stritzler, Margarita; Maguire, Vanina; Ruiz, Oscar; Soldini, Diego Omar; Pascuan, Cecilia Gabriela; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2021-11)Soybean is the most inoculant-consuming crop in the world, carrying strains belonging to the extremely related species Bradyrhizobium japonicum and Bradyrhizobium diazoefficiens. Currently, it is well known that B. japonicum ...