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Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay
Abstract
Antigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was used to synthesise an antigen-biotin conjugate using bovine serum albumin (BSA) as a bridge, and tested this conjugate in an enzymoimmunoassay for clenbuterol detection. The assay included a highly specific antibody and the biotin-avidin amplification system. Its coefficient of variation was lower than 20% in the range of 100–8000 pg per well and the limit
[ver mas...]
Antigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was used to synthesise an antigen-biotin conjugate using bovine serum albumin (BSA) as a bridge, and tested this conjugate in an enzymoimmunoassay for clenbuterol detection. The assay included a highly specific antibody and the biotin-avidin amplification system. Its coefficient of variation was lower than 20% in the range of 100–8000 pg per well and the limit of detection was 6.4 pg. The synthesis of clenbuterol–BSA–biotin conjugate was reproducible, the reaction product easily purified and the amplification capacity could be set by adjusting the clenbuterol:biotin ratio
[Cerrar]
![Thumbnail](/bitstream/handle/20.500.12123/3977/FoodandAgriculturalImmunology-cover.jpg?sequence=3&isAllowed=y)
Author
Spotorno, Viviana Graciela;
Tezón, Jorge Guillermo;
Fuente
Food and Agricultural Immunology 23 (4) : 315-328 (December 2012)
Date
2012-12
Editorial
Taylor & Francis
ISSN
0954-0105
1465-3443
1465-3443
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Derechos de acceso
Abierto
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