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Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the “Zona Alta del Río Mendoza” region in Argentina
Mercado, Laura Analia; Sturm, Maria Elena; Rojo, Maria Cecilia; Ciklic, Ivan Francisco; Martínez, Claudio; Combina, Mariana (Elsevier, 2011-12-15)The “Zona Alta del Río Mendoza” (ZARM) is the major Malbec grape viticulture region of Argentina. The aim of the present study was to explore Saccharomyces cerevisiae biodiversity in ZARM vineyards. Interdelta PCR and RFLP ... -
Construction of low-ethanol-wine yeasts through partial deletion of the Saccharomyces cerevisiae PDC2 gene
Cuello, Raúl Andrés; Flores Montero, Karina Johana; Mercado, Laura Analia; Combina, Mariana; Ciklic, Ivan Francisco (2017)We propose an alternative GMO based strategy to obtain Saccharomyces cerevisiae mutant strains with a slight reduction in their ability to produce ethanol, but with a moderate impact on the yeast metabolism. Through ... -
Effect of low temperature fermentation on the yeast-derived volatile aroma composition and sensory profile in Merlot wines
Massera, Ariel Fernando; Assof, Mariela Vanesa; Sari, Santiago Eduardo; Ciklic, Ivan Francisco; Mercado, Laura Analia; Jofre, Viviana Patricia; Combina, Mariana (Elsevier, 2021-02)Different winemaking practices have been tried to improve wine quality and differentiate wine styles. Low temperature fermentation has been proposed to enhance the aroma profile in young red wines. The aim of this work was ... -
Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing
Ravn, Jonas L.; Manfrão-Netto, João H.C.; Schaubeder, Jana B.; Torello Pianale, Luca; Spirk, Stefan; Ciklic, Ivan Francisco; Geijer, Cecilia (BMC, 2024-03)Background: The abundance of glucuronoxylan (GX) in agricultural and forestry residual side streams positions it as a promising feedstock for microbial conversion into valuable compounds. By engineering strains of the ... -
Generation of a non-transgenic genetically improved yeast strain for wine production from nitrogen-deficient musts
Kessi Pérez, Eduardo Ignacio; Molinet, Jennifer; García, Verónica; Aguilera, Omayra; Cepeda, Fernanda; López, María Eugenia; Sari, Santiago Eduardo; Cuello, Raúl Andrés; Ciklic, Ivan Francisco; Rojo, Cecilia; Combina, Mariana; Araneda, Cristián; Martínez, Claudio (MDPI, 2020)The yeast Saccharomyces cerevisiae is the main species responsible for the process that involves the transformation of grape must into wine, with the initial nitrogen in the grape must being vital for it. One of the main ... -
Hybridization and spore dissection of native wine yeasts for improvement of ethanol resistance and osmotolerance
Sanchez, Maria Laura; Chimeno, Selva Valeria; Mercado, Laura Analia; Ciklic, Ivan Francisco (Springer, 2022-09)Global warming has a significant impact on different viticultural parameters, including grape maturation. An increment of photosynthetic activity generates a rapid accumulation of sugars in the berry, followed by a dehydration ... -
Mutagenesis, screening and isolation of Brettanomyces bruxellensis mutants with reduced 4-ethylphenol production
Alvarez Gaona, Izmari Jasel; Assof, Mariela Vanesa; Jofre, Viviana Patricia; Combina, Mariana; Ciklic, Ivan Francisco (Springer, 2021-01)The use of non-conventional yeast species to obtain interesting flavors and aromas has become a new trend in the fermented beverages industry. Among such species, Brettanomyces bruxellensis (B. bruxellensis) has been ...