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Mostrando ítems 1-20 de 44
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A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
(Sage Publications, 2020-02)Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization ... -
A rapid and affordable amplicon-based method for next generation genome sequencing of the infectious bursal disease virus
(Elsevier, 2023-12)The infectious bursal disease virus (IBDV) causes a severe immunosuppressive disorder in young chickens. IBDV evolution resulted in the emergence of strains with divergent genetic, antigenic, and pathogenic characteristics. ... -
Análisis genómico de girasol: desarrollo de colecciones de ESTs y de una plataforma bioinformática para estudios de expresión de genes candidato en respuestas a estreses abióticos
(Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2006)El girasol es uno de los principales cultivos oleaginosos del mundo por su volumen de producción y composición en ácidos grasos insaturados. En promedio, durante los últimos cinco años, la producción mundial se ha mantenido ... -
Bayesian assessment of the accuracy of a PCR-based rapid diagnostic test for bovine tuberculosis in swine
(Frontiers Media, 2019-06)Infection with the Mycobacterium bovis (M. bovis) causes a disease referred to as bovine tuberculosis (bTB), which affects a wide range of mammal hosts. Many countries have implemented control and eradication plans that ... -
Caracterización taxonómica y análisis de la variabilidad del agente causal del cancro del tallo de la soja en Buenos Aires (2005/2007)
(Gerencia de Comunicación e Imagen Institucional, DNA SICC, INTA, 2011-12)El complejo Diaporthe/Phomopsis es un importante grupo de hongos patógenos de la soja. Dentro del mismo, Diaporthe phaseolorum var. caulivora es uno de los agentes causales del cancro del tallo. Veintitrés aislamientos de ... -
Desarrollo de alternativas diagnósticas de tuberculosis bovina
(Universidad Nacional de Quilmes, 2015-02)En el siguiente trabajo se llevó cabo la puesta a punto y optimización de la técnica LAMP (del inglés loop-mediated isothermal amplification) para la detección de Mycobacterium bovis, agente etiológico de la tuberculosis ... -
Detección de mycobacterium bovis en leche bovina a través de bacteriología y PCR
(FundaVet, 2019-06)La tuberculosis bovina (TB) es una enfermedad infecto-contagiosa de importancia mundial. El agente causal, Mycobacterium bovis, además de afectar al ganado tiene implicancias en salud pública al tratarse de una zoonosis. ... -
Detection of Mycobacterium bovis in Bovine and Bubaline tissues using Nested-PCR for TbD1
(PLOS, 2014-03)In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. ... -
Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
(Mary Ann Liebert Inc., 2012-02)Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we ... -
Detection of Sarcocystis aucheniae in blood of llama using a duplex semi-nested PCR assay and its association with cyst infestation
(Elsevier, 2018)The protozoon Sarcocystis aucheniae is the causative agent of South American camelid (SAC) sarcocystosis. Infections are characterized by the presence of cysts in muscles which are in size and appearance similar to rice ... -
Development and evaluation of a one-step multiplex real-time TaqMan® RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples
(BMC, 2019-04)Background: Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and has a negative impact on equine breeding enterprises worldwide. Among ERVA strains infecting foals, the genotypes G3P[12] and ... -
Development of a droplet digital PCR assay for quantification of the proviral load of bovine leukemia virus
(Sage Publications, 2022-05)Droplet digital PCR (ddPCR) is a highly sensitive tool developed for the detection and quantification of short-sequence variants—a tool that offers unparalleled precision enabling measurement of smaller-fold changes. We ... -
Development of a PCR assay for identification of Neobalantidium coli (Pomajbíková et al., 2013) in Argentina
(2017-12)Neobalantidium coli (Pomajbíková et al., 2013) is a cosmopolitan ciliate which colonizes the intestine of humans and animals. Pigs are the most important host and reservoir for this parasite, although others mammals have ... -
Development of highly sensitive one step-PCR tests for improved detection of B. bigemina and B. bovis
(Elsevier, 2021-08)Bovine babesiosis caused by Babesia bigemina and B. bovis is an economically relevant tick-borne disease distributed over tropical and subtropical world regions. Animals that recover from the clinical disease can remain ... -
Development of RT-qPCR assays for the specific identification of two major genotypes of avian infectious bronchitis virus
(2016)Infectious bronchitis virus (Gammacoronavirus, Coronaviridae) is a genetically variable RNA virus (27.6kb) that causes one of the most persistent respiratory disease in poultry. The virus is classified in genotypes with ... -
Diagnosis of canine leptospirosis: evaluation of two PCR assays in comparison with the microagglutination test = Diagnóstico de leptospirose canina: avaliação de dois ensaios de PCR em comparação com o teste de microaglutinação
(Colegio Brasileiro de Patologia Animal, 2019-04)Canine leptospirosis is definitely diagnosed by demonstrating seroconversion in paired serum samples from the acute and convalescent period by the microagglutination test (MAT). However, the application of a polymerase ... -
Diagnóstico de Entamoeba polecki y su potencial impacto en las condiciones sanitarias de la producción porcina
(Ediciones INTA, 2019-12)En Argentina, la producción porcina constituye una actividad en constante aumento, en particular, para la pequeña agricultura familiar. No obstante, este tipo de producción posee ciertas limitaciones (estructurales y ... -
Diferenciación de serovares de leptospiras patógenas mediante PCR del gen ligB y secuenciación = Differentiation of pathogenic leptospires spp by PCR of ligB gene and sequencing
(Asociación Argentina de Microbiología, 2018)La leptospirosis es la zoonosis de mayor distribución mundial. El objetivo del presente trabajo fue desarrollar una técnica molecular que diferencie leptospiras patógenas. Se amplificó mediante PCR y se secuenció una ... -
First report of pathogenic Leptospira spp. in Tadarida brasiliensis bats (family Molossidae) and Eptesicus furinalis (family Vespertilionidae) of Argentina. New host species in this country? = Primer reporte de Leptospira spp. patógena en murciélagos Tadarida brasiliensis (familia: Molossidae) y Eptesicus furinalis (familia: Vespertilionidae) en Argentina. ¿Nuevas especies hospedadoras en el país?
(Elsevier, 2021-09)Leptospirosis is an endemic disease caused by Leptospira spp., a bacterium that affects animals and humans. In recent years, the number of reports of leptospirosis in wild animals has increased, which highlights the need ...