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Generalized glycogenosis in Brahman-derived breeds: diagnosis and prevalence in Argentina
(Springer, 2019-08)Generalized glycogenosis is a lethal autosomal recessive disease caused by a deficient activity of the acidic 1,4-α-glucosidase enzyme and characterized by an accumulation of glycogen within lysosomes. Three mutations in ... -
Genotipificación de variantes de beta-caseína bovina mediante ACRSPCR- RFLP = Genotyping of bovine beta-casein variants by ACRS-PCR-RFLP
(Consejo de Rectores de Universidades Privadas, 2023-10)Las variantes de la beta-caseína bovina pueden clasificarse en dos grupos, tipo-A1 y tipo-A2, según el aminoácido que esté presente en la posición 67 de la proteína (His, tipo A1; Pro, tipo A2)1. En los últimos años se ha ... -
Highly sensitive nested PCR and rapid immunochromatographic detection of Babesia bovis and Babesia bigemina infection in a cattle herd with acute clinical and fatal cases in Argentina
(Wiley, 2020-07)Bovine babesiosis is a tick‐transmitted haemoparasitic disease caused by Babesia bovis and B. bigemina affecting cattle of tropical and subtropical regions around the world. Pathogens are transmitted by the tick vector ... -
International interlaboratory validation of a nested PCR for molecular detection of Babesia bovis and Babesia bigemina, causative agents of bovine babesiosis
(Elsevier, 2022-04)Babesia bovis and B. bigemina are tick-transmitted parasites causing bovine babesiosis, characterized by significant morbidity and mortality leading to economic losses to the livestock industry in tropical and subtropical ... -
L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus
(2019-11)Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive ... -
Manual sobre diagnóstico molecular de leptospirosis
(INTA Ediciones, 2022)Este manual es una recopilación sobre las herramientas moleculares de diagnóstico y caracterización de cepas aisladas. Este trabajo es resultado de la integración de más de 10 años de trabajo durante mi tesis doctoral y ... -
Microscopic and PCR-based detection of microsporidia spores in human stool samples = Detección microscópica y molecular de esporas de microsporidios en muestras de heces humanas
(Elsevier, 2020-06)Microsporidia are obligate intracellular fungi with a remarkable ability to infect a wide range of invertebrate and vertebrate hosts. Namely, Enterocytozoon bieneusi is the most frequently microsporidia reported worldwide, ... -
Modification of a previously patented method to unequivocally score A2-like and A1-like bovine β-casein variants
(Elsevier, 2023-04)A growing interest in the production and commercialization of A2 cow's milk has been observed in many countries in the last few years due to the beneficial properties for human health attributed to A2 β-casein variant. ... -
Molecular diagnosis of Leishmania spp. in dogs of a subtropical locality of Argentina
(Wiley, 2020-07)Leishmaniosis is a tropical and subtropical vector‐borne disease caused by hemoparasites of the genus Leishmania. The disease can infect humans, as well as domestic and wildlife animals. Dogs are the main reservoir for L. ... -
Multiplex PCR followed by restriction length polymorphism analysis for the subtyping of bovine herpesvirus 5 isolates
(BMC, 2013-06)Background: Several types and subtypes of bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) have been associated to different clinical conditions of cattle, making type/subtype differentiation essential to understand the ... -
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
(Elsevier, 2018-07)Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are ... -
Optimization and comparative analysis of LAMP and PCR techniques for the detection of leptospiral DNA in Golden Syrian hamsters
(Springer, 2023-08)Leptospirosis is a zoonotic disease with significant public health and economic impact worldwide. Rapid and accurate diagnosis is essential for effective prevention and treatment. This study optimized a loop-mediated ... -
Pandemic (H1N1) 2009 outbreak on pig farm, Argentina
(Centers for Disease Control and Prevention, 2010-02)In June–July 2009, an outbreak of pandemic (H1N1) 2009 infection occurred on a pig farm in Argentina. Molecular analysis indicated that the virus was genetically related to the pandemic (H1N1) 2009 influenza virus strain. ... -
Preparation of a working seed lot of BCG and quality control by PCR genotyping
(Asociación Argentina de Microbiología, 2010-04)The bacillus Calmette-Guérin (BCG) was obtained in 1920 after successive passages leading to the attenuation of a Mycobacterium bovis strain. For the following 40 years, BCG had been replicated, resulting in substrains ... -
Puesta a punto de una PCR en tiempo real para la detección de Senecavirus A
(Universidad Nacional del Centro de la Provincia de Buenos Aires, 2023-08)Senecavirus A (SVA). Familia : Picornaviridae Única especie del género Senecavirus. No envuelto ARN + monocatenario. La Organización Mundial de Sanidad Animal ( lo considera de gran importancia debido a que sus signos ... -
Quadruplex real-time TaqMan® RT-qPCR assay for differentiation of equine group A and B rotaviruses and Identification of group A G3 and G14 genotypes
(MDPI, 2023-07)Equine rotavirus A (ERVA) is the leading cause of diarrhea in foals, with G3P[12] and G14P[12] genotypes being the most prevalent. Recently, equine G3-like RVA was recognized as an emerging infection in children, and a ... -
Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine
(Elsevier, 2013-10)In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing ... -
Rapid detection of equine infectious anaemia virus nucleic acid by insulated isothermal RT-PCR assay to aid diagnosis under field conditions
(Wiley, 2018)Background: Control of equine infectious anaemia (EIA) currently depends on serological diagnosis of infected equids. However, recently infected equids may not produce detectable anti-EIAV antibodies up to 157 days post ... -
Tuberculosis bovina : transmisibilidad de cepas de Mycobacterium bovis y detección de micobacterias en menudencias comercializadas en bocas de expendio de carne
(Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, 2019)La tuberculosis bovina, causada por Mycobacterium bovis (M. bovis), es endémica en la Argentina. Su presencia en el ganado bovino representa un riesgo para la salud humana debido a su carácter zoonótico, pudiendo además ... -
Tuberculosis in swine co-infected with Mycobacterium avium subsp. hominissuis and Mycobacterium bovis in a cluster from Argentina
(Cambridge University Press, 2015-04)In Argentina little is known about the epidemiology of tuberculosis (TB) infection in swine. We characterized the epidemiological dynamics of Mycobacterium avium complex (MAC) infection in a swine population of Argentina ...