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Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
Resumen
This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent protein (GFP) as a selection and reporter agents, respectively. All transgenic isolates were mitotically stable in two independent experiments and polymerase
[ver mas...]
This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent protein (GFP) as a selection and reporter agents, respectively. All transgenic isolates were mitotically stable in two independent experiments and polymerase chain reaction with hygromycin B resistance primers confirmed successful T-DNA integration into the fungal genome. Plant-fungus interaction studies, including pathogenicity, latency, and endophytism, as well as further studies of random and targeted mutagenesis will be possible with GFP-expressing isolates of D. caulivora and other species in the Diaporthe / Phomopsis complex.
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Fuente
Journal of Microbiological Methods 184 : 106197 (May 2021)
Fecha
2021-03-10
Editorial
Elsevier
ISSN
0167-7012
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pdf
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artículo
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(ver más)
INTA/PNPV-1135024/AR./Defensa en plantas contra fitopatógenos.
INTA/PNCYO-1127034/AR./Evaluación y desarrollo de sistemas de manejo integrado de las plagas en cultivos de cereales y oleaginosas.
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Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)