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Mostrando ítems 1-15 de 15

    • Cold sweetening diversity in Andean potato germplasm from Argentina 

      Colman, Silvana; Massa, Gabriela Alejandra; Carboni, Martín Federico; Feingold, Sergio Enrique (Wiley Online Library, 2018-04-02)
      BACKGROUND: Cold induced sweetening (CIS) is the accumulation of sucrose and reducing sugars in potato tubers at low temperatures. This process is central for the potato processing industry. During potato chip and French ...

    • Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato 

      Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Anderson, Mariette; Decima Oneto, Cecilia Andrea; Turesson, Helle; Storani, Leonardo; Olsson, Niklas; Fält, Ann Sofie; Hofvander, Per; Feingold, Sergio Enrique (Keystone Symposia on Molecular and Cellular Biology, 2021-03)
      StPPO2 gene editing was analyzed by Agrobacterium mediated transformation with CR-PPO vector, ribonucleoprotein complexes (RNP-PPO) transfection to protoplasts, and CR-PPO transient expression in protoplasts, yielding ...

    • Comparative potato genome editing: Agrobacterium tumefaciens-mediated transformation and protoplasts transfection delivery of CRISPR/Cas9 components directed to StPPO2 gene 

      Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Anderson, Mariette; Decima Oneto, Cecilia Andrea; Turesson, Helle; Olsson, Niklas; Fält, Ann Sofie; Hofvander, Per; Feingold, Sergio Enrique (Springer Netherlands, 2021-01-25)
      Delivery of the CRISPR/Cas9 components to the plant cells is a key step in its application as a genome editing tool. Here, we compared Agrobacterium-mediated transformation and protoplast transfection with CRISPR/Cas9 ...

    • CRISPR/Cas9 for potato functional genomics and breeding 

      Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Andersson, Mariette; Storani, Leonardo; Olsson, Niklas; Decima Oneto, Cecilia Andrea; Hofvander, Per; Feingold, Sergio Enrique (Humana Press (Springer), 2023-03-31)
      Cultivated potato (Solanum tuberosum L.) is one of the most important staple food crops worldwide. Its tetraploid and highly heterozygous nature pose a great challenge to its basic research and trait improvement through ...

    • Edición génica con el sistema CRISPR/Cas9: historia de su descubrimiento y alcances en agricultura 

      Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Feingold, Sergio Enrique (Universidad de Los Andes, Venezuela, 2021-04)
      La conversión del sistema inmune procariota CRISPR/Cas9 en una herramienta molecular para edición génica representa el avance tecnológico más destacado de la última década, y ha revolucionado tanto la investigación básica ...

    • Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora 

      Montoya, Marina Rosa; Massa, Gabriela Alejandra; Colabelli, Mabel Noemí; Ridao, Azucena (Elsevier, 2021-03-10)
      This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium ...

    • Efficient CRISPR/Cas9 genome editing in alfalfa using a public germplasm 

      Bottero, Ana Emilia; Massa, Gabriela Alejandra; Gonzalez, Matías Nicolás; Stritzler, Margarita; Tajima, Hiromi; Gomez, Maria Cristina; Frare, Romina Alejandra; Feingold, Sergio Enrique; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Frontiers Media, 2021-07)
      Because its ability to acquire large amounts of nitrogen by symbiosis, tetraploid alfalfa is the main source of vegetable proteins in meat and milk production systems in temperate regions. Alfalfa cultivation also adds ...

    • Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina 

      Colavita, Monica; Lancia, Marcos; Feingold, Sergio Enrique; Massa, Gabriela Alejandra (Springer Netherlands, 2017-06)
      In order to make a first approach in the identification of the genetic diversity of Potato virus Y (PVY) in Argentina, 46 PVY isolates from different potato growing regions of Argentina were characterized both, biological ...

    • Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Solanum tuberosum): Generating New Tools to Improve the Resistance of Plants to Abiotic Stress 

      Norero, Natalia Sigrid; Rey Burusco, María Florencia; D’Ippólito, Sebastián; Decima Oneto, Cecilia Andrea; Massa, Gabriela Alejandra; Castellote, Martín Alfredo; Feingold, Sergio Enrique; Guevara, María Gabriela (Multidisciplinary Digital Publishing Institute, MDPI, 2022-02)
      Aspartic proteases are proteolytic enzymes widely distributed in living organisms and viruses. Although they have been extensively studied in many plant species, they are poorly described in potatoes. The present study ...

    • Identificación y caracterización de Aspartil proteasas en Solanum tuberosum relacionadas a diferentes tipos de estrés 

      Norero, Natalia Sigrid; Rey Burusco, Maria Florencia; D`Ippólito, S.; Castellote, Martín Alfredo; Decima Oneto, Cecilia Andrea; Massa, Gabriela Alejandra; Feingold, Sergio Enrique; Guevara, Gabriela (Secretaria de Ciencia y Tecnología, Universidad Nacional de Mar del Plata, 2020-10)
      En este trabajo, realizamos un análisis del genoma de S. tuberosum para explorar las funciones potenciales de las Aspartil proteasas (AP, EC 3.4.23). Las APs son enzimas proteolíticas con funciones muy diversas que se ...

    • New Frontiers in Potato Breeding: Tinkering with Reproductive Genes and Apomixis 

      Hojsgaard, Diego; Feingold, Sergio Enrique; Massa, Gabriela Alejandra; Bradshaw, John (Multidisciplinary Digital Publishing Institute, MDPI, 2024-05)
      Potato is the most important non-cereal crop worldwide, and, yet, genetic gains in potato have been traditionally delayed by the crop’s biology, mostly the genetic heterozygosity of autotetraploid cultivars and the intricacies ...

    • Obtención de plantas de papa sobrexpresantes de la aspartil proteasa StAP3 para incrementar la tolerancia a sequía 

      Decima Oneto, Cecilia Andrea; D`Ippólito, S.; Massa, Gabriela Alejandra; Rey, María Florencia; Norero, Natalia Sigrid; Feingold, Sergio Enrique; Guevara, Gabriela (Secretaria de Ciencia y Tecnología, Universidad Nacional de Mar del Plata, 2020-10)
      Una de las consecuencias del cambio climático es el estrés generado por la disminución de disponibilidad de agua (Leakey et al., 2009). La planta de papa, que representa una de las fuentes alimenticias más importantes para ...

    • Reduced enzymatic browning in potato tubers by specific editing of a Polyphenol oxidase gene via Ribonucleoprotein complexes delivery of the CRISPR/Cas9 System. 

      Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Andersson, Mariette; Turesson, Helle; Olsson, Niklas; Fält, Ann-Sofie; Storani, Leonardo; Decima Oneto, Cecilia Andrea; Hofvander, Per; Feingold, Sergio Enrique (Frontiers Media, Switzerland, 2020-01-09)
      Polyphenol Oxidases (PPOs) catalyze the conversion of phenolic substrates to quinones, leading to the formation of dark-colored precipitates in fruits and vegetables. This process, known as enzymatic browning, is the cause ...

    • State of the art of genetic engineering in potato: from the first report to its future potential 

      Nahirñak, Vanesa; Almasia, Natalia Ines; Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Decima Oneto, Cecilia Andrea; Feingold, Sergio Enrique; Hopp, Horacio Esteban; Vazquez Rovere, Cecilia (Frontiers Media, 2022-01)
      Potato (Solanum tuberosum L.) is a crop of world importance that produces tubers of high nutritional quality. It is considered one of the promising crops to overcome the challenges of poverty and hunger worldwide. However, ...

    • Transformación eficiente de Solanum tuberosum cv. Spunta mediada por Agrobacterium tumefaciens utilizando higromicina como agente selectivo 

      Decima Oneto, Cecilia Andrea; Coronel, Joel; Storani, Leonardo; Gonzalez, Matías Nicolás; Feingold, Sergio Enrique; Massa, Gabriela Alejandra (Ediciones INTA, 2020-08)
      La transformación genética de plantas es una herramienta biotecnológica de alto impacto en el mejoramiento vegetal que brinda la posibilidad de obtener nuevos cultivos que se adapten a las demandas de producción y de ...