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Abstract
Dendritic cells serve as the main immune cells that trigger the immune response. We developed a simple and cost-effective nanovaccine platform based on the α1′,2-mannobiose derivative for dendritic cell targeting. In previous work, we have formulated the α1,2-mannobiose-based nanovaccine platform with plasmid DNA and tested it in cattle against BoHV-1 infection. There, we have shown that the dendritic cell targeting using this nanovaccine platform in vivo [ver mas...]
dc.contributor.authorPappalardo, Juan Sebastián
dc.contributor.authorSalmaso, Stefano
dc.contributor.authorLevchenko, Tatyana S.
dc.contributor.authorMastrotto, Francesca
dc.contributor.authorBersani, Sara
dc.contributor.authorVermeulen, Monica
dc.contributor.authorGhersa, Federica
dc.contributor.authorQuattrocchi, Valeria
dc.contributor.authorZamorano, Patricia Ines
dc.contributor.authorHartner, William C.
dc.contributor.authorToniutti, Micaela
dc.contributor.authorMusacchio, Tiziana
dc.contributor.authorTorchilin, Vladimir P.
dc.date.accessioned2021-07-06T17:11:03Z
dc.date.available2021-07-06T17:11:03Z
dc.date.issued2021-07
dc.identifier.issn1543-8384
dc.identifier.otherhttps://doi.org/10.1021/acs.molpharmaceut.1c00048
dc.identifier.urihttp://hdl.handle.net/20.500.12123/9742
dc.identifier.urihttps://pubs.acs.org/doi/10.1021/acs.molpharmaceut.1c00048
dc.description.abstractDendritic cells serve as the main immune cells that trigger the immune response. We developed a simple and cost-effective nanovaccine platform based on the α1′,2-mannobiose derivative for dendritic cell targeting. In previous work, we have formulated the α1,2-mannobiose-based nanovaccine platform with plasmid DNA and tested it in cattle against BoHV-1 infection. There, we have shown that the dendritic cell targeting using this nanovaccine platform in vivo can boost the immunogenicity, resulting in a longlasting immunity. In this work, we aim to characterize the α1′,2-mannobiose derivative, which is key in the nanovaccine platform. This DC-targeting strategy takes advantage of the specific receptor known as DC-SIGN and exploits its capacity to bind α1,2-mannobiose that is present at terminal ends of oligosaccharides in certain viruses, bacteria, and other pathogens. The oxidative conjugation of α1′,2-mannobiose to NH2-PEG2kDa-DSPE allowed us to preserve the chemical structure of the non-reducing mannose of the disaccharide and the OH groups and the stereochemistry of all carbons of the reducing mannose involved in the binding to DC-SIGN. Here, we show specific targeting to DC-SIGN of decorated micelles incubated with the Raji/DC-SIGN cell line and uptake of targeted liposomes that took place in human, bovine, mouse, and teleost fish DCs in vitro, by flow cytometry. Specific targeting was found in all cultures, demonstrating a species-non-specific avidity for this ligand, which opens up the possibility of using this nanoplatform to develop new vaccines for various species, including humans.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherACS Publicationses_AR
dc.relationinfo:eu-repograntAgreement/INTA/PNBIO-1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular.
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceMolecular pharmaceutics 18 (7) : 2540-2555 (Julio 2021)es_AR
dc.subjectNanomedicinaes_AR
dc.subjectNanomedicineeng
dc.subjectVacunaes_AR
dc.subjectVaccineseng
dc.subjectAntígenoses_AR
dc.subjectAntigenseng
dc.subjectRespuesta Inmunológicaes_AR
dc.subjectImmune Responseeng
dc.subject.otherNanovacunaes_AR
dc.subject.otherCélulas Dendríticases_AR
dc.titleCharacterization of a Nanovaccine Platform Based on an α1,2- Mannobiose Derivative Shows Species-non-specific Targeting to Human, Bovine, Mouse, and Teleost Fish Dendritic Cellses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEstación Experimental Agropecuaria Barilochees_AR
dc.description.filFil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentinaes_AR
dc.description.filFil: Pappalardo, Juan Sebastián. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentinaes_AR
dc.description.filFil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.description.filFil: Pappalardo, Juan Sebastián. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidoses_AR
dc.description.filFil: Salmaso, Stefano. University of Padova. School of Medicine. Department of Pharmaceutical and Pharmacological Sciences; Italiaes_AR
dc.description.filFil: Levchenko, Tatyana S. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidoses_AR
dc.description.filFil: Mastrotto, Francesca. University of Padova. School of Medicine. Department of Pharmaceutical and Pharmacological Sciences; Italiaes_AR
dc.description.filFil: Bersani, Sara. University of Padova. School of Medicine. Department of Pharmaceutical and Pharmacological Sciences; Italiaes_AR
dc.description.filFil: Vermeulen, Monica. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentinaes_AR
dc.description.filFil: Vermeulen, Monica. Academia Nacional de Medicina. Instituto de Medicina Experimental; Argentinaes_AR
dc.description.filFil: Ghersa, Federica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentinaes_AR
dc.description.filFil: Ghersa, Federica. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentinaes_AR
dc.description.filFil: Ghersa, Federica. Universidad Nacional del Comahue. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentinaes_AR
dc.description.filFil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.description.filFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.description.filFil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentinaes_AR
dc.description.filFil: Hartner, William C. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidoses_AR
dc.description.filFil: Toniutti, Micaela. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidoses_AR
dc.description.filFil: Musacchio, Tiziana. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidoses_AR
dc.description.filFil: Torchilin, Vladimir P. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidoses_AR
dc.subtypecientifico


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