Mostrar el registro sencillo del ítem
resumen
Resumen
Dendritic cells serve as the main immune cells that trigger the immune response. We developed a simple and cost-effective nanovaccine platform based on the α1′,2-mannobiose derivative for dendritic cell targeting. In previous work, we have formulated the α1,2-mannobiose-based nanovaccine platform with plasmid DNA and tested it in cattle against BoHV-1 infection. There, we have shown that the dendritic cell targeting using this nanovaccine platform in vivo
[ver mas...]
dc.contributor.author | Pappalardo, Juan Sebastián | |
dc.contributor.author | Salmaso, Stefano | |
dc.contributor.author | Levchenko, Tatyana S. | |
dc.contributor.author | Mastrotto, Francesca | |
dc.contributor.author | Bersani, Sara | |
dc.contributor.author | Vermeulen, Monica | |
dc.contributor.author | Ghersa, Federica | |
dc.contributor.author | Quattrocchi, Valeria | |
dc.contributor.author | Zamorano, Patricia Ines | |
dc.contributor.author | Hartner, William C. | |
dc.contributor.author | Toniutti, Micaela | |
dc.contributor.author | Musacchio, Tiziana | |
dc.contributor.author | Torchilin, Vladimir P. | |
dc.date.accessioned | 2021-07-06T17:11:03Z | |
dc.date.available | 2021-07-06T17:11:03Z | |
dc.date.issued | 2021-07 | |
dc.identifier.issn | 1543-8384 | |
dc.identifier.other | https://doi.org/10.1021/acs.molpharmaceut.1c00048 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/9742 | |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.1c00048 | |
dc.description.abstract | Dendritic cells serve as the main immune cells that trigger the immune response. We developed a simple and cost-effective nanovaccine platform based on the α1′,2-mannobiose derivative for dendritic cell targeting. In previous work, we have formulated the α1,2-mannobiose-based nanovaccine platform with plasmid DNA and tested it in cattle against BoHV-1 infection. There, we have shown that the dendritic cell targeting using this nanovaccine platform in vivo can boost the immunogenicity, resulting in a longlasting immunity. In this work, we aim to characterize the α1′,2-mannobiose derivative, which is key in the nanovaccine platform. This DC-targeting strategy takes advantage of the specific receptor known as DC-SIGN and exploits its capacity to bind α1,2-mannobiose that is present at terminal ends of oligosaccharides in certain viruses, bacteria, and other pathogens. The oxidative conjugation of α1′,2-mannobiose to NH2-PEG2kDa-DSPE allowed us to preserve the chemical structure of the non-reducing mannose of the disaccharide and the OH groups and the stereochemistry of all carbons of the reducing mannose involved in the binding to DC-SIGN. Here, we show specific targeting to DC-SIGN of decorated micelles incubated with the Raji/DC-SIGN cell line and uptake of targeted liposomes that took place in human, bovine, mouse, and teleost fish DCs in vitro, by flow cytometry. Specific targeting was found in all cultures, demonstrating a species-non-specific avidity for this ligand, which opens up the possibility of using this nanoplatform to develop new vaccines for various species, including humans. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | ACS Publications | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/PNBIO-1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular. | |
dc.relation | info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública | |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Molecular pharmaceutics 18 (7) : 2540-2555 (Julio 2021) | es_AR |
dc.subject | Nanomedicina | es_AR |
dc.subject | Nanomedicine | eng |
dc.subject | Vacuna | es_AR |
dc.subject | Vaccines | eng |
dc.subject | Antígenos | es_AR |
dc.subject | Antigens | eng |
dc.subject | Respuesta Inmunológica | es_AR |
dc.subject | Immune Response | eng |
dc.subject.other | Nanovacuna | es_AR |
dc.subject.other | Células Dendríticas | es_AR |
dc.title | Characterization of a Nanovaccine Platform Based on an α1,2- Mannobiose Derivative Shows Species-non-specific Targeting to Human, Bovine, Mouse, and Teleost Fish Dendritic Cells | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | Estación Experimental Agropecuaria Bariloche | es_AR |
dc.description.fil | Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina | es_AR |
dc.description.fil | Fil: Pappalardo, Juan Sebastián. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina | es_AR |
dc.description.fil | Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina | es_AR |
dc.description.fil | Fil: Pappalardo, Juan Sebastián. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos | es_AR |
dc.description.fil | Fil: Salmaso, Stefano. University of Padova. School of Medicine. Department of Pharmaceutical and Pharmacological Sciences; Italia | es_AR |
dc.description.fil | Fil: Levchenko, Tatyana S. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos | es_AR |
dc.description.fil | Fil: Mastrotto, Francesca. University of Padova. School of Medicine. Department of Pharmaceutical and Pharmacological Sciences; Italia | es_AR |
dc.description.fil | Fil: Bersani, Sara. University of Padova. School of Medicine. Department of Pharmaceutical and Pharmacological Sciences; Italia | es_AR |
dc.description.fil | Fil: Vermeulen, Monica. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina | es_AR |
dc.description.fil | Fil: Vermeulen, Monica. Academia Nacional de Medicina. Instituto de Medicina Experimental; Argentina | es_AR |
dc.description.fil | Fil: Ghersa, Federica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina | es_AR |
dc.description.fil | Fil: Ghersa, Federica. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina | es_AR |
dc.description.fil | Fil: Ghersa, Federica. Universidad Nacional del Comahue. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina | es_AR |
dc.description.fil | Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina | es_AR |
dc.description.fil | Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina | es_AR |
dc.description.fil | Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina | es_AR |
dc.description.fil | Fil: Hartner, William C. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos | es_AR |
dc.description.fil | Fil: Toniutti, Micaela. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos | es_AR |
dc.description.fil | Fil: Musacchio, Tiziana. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos | es_AR |
dc.description.fil | Fil: Torchilin, Vladimir P. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos | es_AR |
dc.subtype | cientifico |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
common
-
Artículos científicos [562]