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Abstract
Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., [ver mas...]
dc.contributor.authorCabrera Mederos, Dariel
dc.contributor.authorDal Zotto, Angelica
dc.contributor.authorGaldeano, E.
dc.contributor.authorPortal, O.
dc.contributor.authorGiolitti, Fabian
dc.date.accessioned2021-07-01T12:16:17Z
dc.date.available2021-07-01T12:16:17Z
dc.date.issued2016-06-20
dc.identifier.issn1125-4653
dc.identifier.issn2239-7264 (online)
dc.identifier.otherhttp://dx.doi.org/10.4454/JPP.V98I3.017
dc.identifier.urihttp://hdl.handle.net/20.500.12123/9708
dc.identifier.urihttp://www.sipav.org/main/jpp/index.php/jpp/article/view/3691
dc.description.abstractPapaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5’-TCCAAGAATGAAGCTGTGGACGCTGGTT-3’ and BoCP_Rev 5’-TYAGTTGCGCATACCCAGGAGAGAGT-3’. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherDipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Baries_AR
dc.relationinfo:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.es_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceJournal of Plant Pathology 98 (3) : 687 (2016)es_AR
dc.subjectCarica Papayaes_AR
dc.subjectViruseseng
dc.subjectArgentinaes_AR
dc.subjectCarica Papayaes_AR
dc.subjectPotyviruses_AR
dc.subjectPapaya Ringspot Viruseng
dc.subjectPapayaseng
dc.subjectViruses_AR
dc.subject.otherPapayaes_AR
dc.titleFirst report of Papaya ringspot virus infecting Carica papaya in Argentinaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Patología Vegetales_AR
dc.description.filFil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.description.filFil: Galdeano, Ernestina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentinaes_AR
dc.description.filFil: Galdeano, Ernestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentinaes_AR
dc.description.filFil: Portal, O. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias; Cubaes_AR
dc.description.filFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentinaes_AR
dc.subtypecientifico


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