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Abstract
Cellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous [ver mas...]
dc.contributor.authorCanal, Ana María
dc.contributor.authorPezzone, Natalia
dc.contributor.authorCataldi, Angel Adrian
dc.contributor.authorZumarraga, Martin Jose
dc.contributor.authorLarzabal, Mariano
dc.contributor.authorGarbaccio, Sergio Gabriel
dc.contributor.authorFernández, Analía
dc.contributor.authorDominguez, Lucas
dc.contributor.authorAranaz, Alicia
dc.contributor.authorRodriguez-Bertos, Antonio
dc.date.accessioned2017-08-11T14:13:26Z
dc.date.available2017-08-11T14:13:26Z
dc.date.issued2017
dc.identifier.issn0034-5288
dc.identifier.otherhttps://doi.org/10.1016/j.rvsc.2016.10.006
dc.identifier.urihttp://hdl.handle.net/20.500.12123/956
dc.identifier.urihttp://ac.els-cdn.com/S0034528816304593/1-s2.0-S0034528816304593-main.pdf?_tid=6259051a-7e9f-11e7-acab-00000aab0f6c&acdnat=1502461047_a4e6d0901a58f8bf245b3583447246aa
dc.description.abstractCellular immune response was evaluated in lymph nodes and lung with different granulomatous lesions from cattle naturally infected with Mycobacterium bovis. For this purpose, we assessed pro-inflammatory and anti-inflammatory cytokines by immunohistochemical assays. Immunoreaction was observed for all the cytokines analyzed. Fourteen animals displayed advanced stage IV granulomas, with intense immunoreactivity to IFN-γ and TGF-β in areas of caseous necrosis, macrophages and lymphocytes. Seven animals showed stage III granuloma, with high immunoreactivity to IFN-γ (average of 44.5% immunoreactive cells) and moderate to TNF-αand to the anti-inflammatory cytokines IL-10 and TGF-β, in relation to the proliferation of fibroblasts in granuloma periphery We found satellite stage I granulomas in 4 bovines and stage II granulomas in 2 bovines, which exhibited low immunostaining response (‐13%). Cytokine expression in stage III and IV granulomas was significant, with predominance of immunoreactivity to IFN-γ, thus suggesting a strong, longstanding local immune response mediated by macrophages and epithelioid cells. In addition, these two stages displayed lower reactivity to IL-10; which suggests a deficit of anti-inflammatory cytokines, suppressed immunity and persistence of the infection. High expression of TGF-β could indicate a chronic processwith greater tissue damage and fibrosis. Numerous bacilli observed in necrotic areas in stage III and IV granulomas with low expression of IL-1β suggest failure in the immune response with bacterial multiplication. In this study, evidence of in situ presence of cytokines demonstrates these cytokines are involved in the development and evolution of bovine tuberculosis granulomas.
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceResearch in veterinary science 110 : 34-39. (2017)eng
dc.subjectEnfermedades de los Animales
dc.subjectMycobacterium bovis
dc.subjectAnimal Diseaseseng
dc.subjectGanado Bovino
dc.subjectCattleeng
dc.subjectCytokineseng
dc.subjectCitoquinas
dc.subjectInfección por Mycobacterium bovis
dc.subjectMycobacterium bovis infectionseng
dc.titleImmunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infectioneng
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInst. de Biotecnología
dc.gic152932
dc.description.filFil: Canal, Ana María. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina
dc.description.filFil: Pezzone, Natalia. Santa Fe. Ministerio de la Producción. Sub-dirección de Ganadería y Sanidad Animal; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Laboratorio de Anatomía Patológica; Argentina
dc.description.filFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
dc.description.filFil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
dc.description.filFil: Fernández, Analía Entre Ríos. Secretaría de Producción. Dirección General de Producción Animal; Argentina
dc.description.filFil: Dominguez, Lucas. Universidad Complutense. Centro de Vigilancia Sanitaria Veterinaria; España. Universidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España
dc.description.filFil: Aranaz, AliciaUniversidad Complutense. Facultad de Veterinaria. Departamento de Sanidad Animal; España
dc.description.filFil: Rodriguez-Bertos, Antonio. Universidad Complutense. Facultad de Veterinaria. Departamento de Medicina Interna y Cirugía; España
dc.subtypecientifico


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