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Mal de Río Cuarto virus (MRCV) is a member of the Fijivirus genus, within the Reoviridae family, that repli-cates and assembles in cytoplasmic inclusion bodies called viroplasms. In this study, we investigatedinteractions between ten MRCV proteins by yeast two-hybrid (Y2H) assays and identified interactionsof non-structural proteins P6/P6, P9-2/P9-2 and P6/P9-1. P9-1 and P6 are the major and minor compo-nents of the viroplasms respectively, whereas P9-2
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dc.contributor.author | Llauger, Gabriela | |
dc.contributor.author | De Haro, Luis Alejandro | |
dc.contributor.author | Alfonso, Victoria | |
dc.contributor.author | Del Vas, Mariana | |
dc.date.accessioned | 2017-08-11T13:05:10Z | |
dc.date.available | 2017-08-11T13:05:10Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0168-1702 | |
dc.identifier.other | https://doi.org/10.1016/j.virusres.2017.01.002 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/954 | |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0168170216307195?via%3Dihub | |
dc.description.abstract | Mal de Río Cuarto virus (MRCV) is a member of the Fijivirus genus, within the Reoviridae family, that repli-cates and assembles in cytoplasmic inclusion bodies called viroplasms. In this study, we investigatedinteractions between ten MRCV proteins by yeast two-hybrid (Y2H) assays and identified interactionsof non-structural proteins P6/P6, P9-2/P9-2 and P6/P9-1. P9-1 and P6 are the major and minor compo-nents of the viroplasms respectively, whereas P9-2 is an N-glycosylated membrane protein of unknownfunction. Interactions involving P6 and P9-1 were confirmed by bimolecular fluorescence complementa-tion (BiFC) in rice protoplasts. We demonstrated that a region including a predicted coiled-coil domainwithin the C-terminal moiety of P6 was necessary for P6/P6 and P6/P9-1 interactions. In turn, a shortC-terminal arm was necessary for the previously reported P9-1 self-interaction. Transient expression ofthese proteins by agroinfiltration of Nicotiana benthamiana leaves showed very low accumulation levelsand further in silico analyses allowed us to identify conserved PEST degradation sequences [rich in pro-line (P), glutamic acid (E), serine (S), and threonine (T)] within P6 and P9-1. The removal of these PESTsequences resulted in a significant increase of the accumulation of both proteins. | |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.rights | info:eu-repo/semantics/restrictedAccess | eng |
dc.source | Virus research 230 :19-28. (2017) | eng |
dc.subject | Fitomejoramiento | |
dc.subject | Enfermedades de las Plantas | |
dc.subject | Plant Breeding | eng |
dc.subject | Plant Diseases | eng |
dc.subject | Plant viruses | eng |
dc.subject | Virus de las Plantas | |
dc.subject | Identificación | |
dc.subject | Identification | eng |
dc.subject.other | Mal de Río Cuarto | |
dc.title | Interaction of Mal de Río Cuarto virus (Fijivirus genus) proteins and identification of putative factors determining viroplasm formation and decay | eng |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.description.origen | Inst. de Biotecnología | |
dc.gic | 152934 | |
dc.description.fil | Fil: Llauger, GabrielaInstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | |
dc.description.fil | Fil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.description.fil | Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.description.fil | Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.subtype | cientifico |
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