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Abstract
Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. [ver mas...]
dc.contributor.authorImperiale, Belen Rocio
dc.contributor.authorMoyano, Roberto Damian
dc.contributor.authorDi Giulio, A.B.
dc.contributor.authorRomero, M.A.
dc.contributor.authorAlvarado Pinedo, Maria Fiorella
dc.contributor.authorSantangelo, María De La Paz
dc.contributor.authorTravería, G.E.
dc.contributor.authorMorcillo, N.S.
dc.contributor.authorRomano, Maria Isabel
dc.coverage.spatialArgentina (nation)
dc.date.accessioned2017-08-10T15:48:13Z
dc.date.available2017-08-10T15:48:13Z
dc.date.issued2017-05
dc.identifier.issn0950-2688 (Print)
dc.identifier.issn1469-4409 (Online)
dc.identifier.otherhttps://doi.org/10.1017/S0950268817000139
dc.identifier.urihttp://hdl.handle.net/20.500.12123/947
dc.identifier.urihttps://www.cambridge.org/core/journals/epidemiology-and-infection/article/genetic-diversity-of-mycobacterium-avium-complex-strains-isolated-in-argentina-by-miruvntr/5C0EF5C3EFC75989893FD6B6631E9962
dc.description.abstractMycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unitvariable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours. inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceEpidemiology and infection 145 (7) : 1382-1391. (2017)eng
dc.subjectGenética
dc.subjectGeneticseng
dc.subjectMycobacterium avium
dc.subjectGenetic Variationeng
dc.subjectVariación Genética
dc.subject.otherDiversidad Genética
dc.titleGenetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTReng
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInst. de Biotecnología
dc.gic154123
dc.description.filFil: Imperiale, Belen Rocio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Di Giulio, A.B. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; San Fernando, Argentina
dc.description.filFil: Romero, M.A. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina
dc.description.filFil: Alvarado Pinedo, Maria Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina
dc.description.filFil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.filFil: Travería, G.E. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina
dc.description.filFil: Morcillo, Nora S.Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
dc.description.filFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.subtypecientifico


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