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Abstract
Staphylococcus aureus is one of the most prevalent pathogens isolated from bovine mastitis, causing chronic intramammary infections (IMI) that limit profitable dairying. The course of infection is often associated with factors both related to the host and the bacterium. Aims of this study were to select S. aureus isolates from bovine IMI with different genotypic profiles harboring genes involved in adherence and biofilm production, to determine the [ver mas...]
dc.contributor.authorPereyra, Elizabet Amanda Lorena
dc.contributor.authorRenna, María Sol
dc.contributor.authorPicech, Florencia
dc.contributor.authorBaravalle, Celina
dc.contributor.authorAndreotti, Carolina Soledad
dc.contributor.authorRussi, Romina
dc.contributor.authorCalvinho, Luis Fernando
dc.contributor.authorDiez, Cristina
dc.contributor.authorDallard, Bibiana Elisabet
dc.date.accessioned2017-08-08T12:32:06Z
dc.date.available2017-08-08T12:32:06Z
dc.date.issued2016
dc.identifier.issn0378-1135
dc.identifier.otherhttps://doi.org/10.1016/j.vetmic.2015.12.002
dc.identifier.urihttp://hdl.handle.net/20.500.12123/919
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0378113515301085?via%3Dihub
dc.description.abstractStaphylococcus aureus is one of the most prevalent pathogens isolated from bovine mastitis, causing chronic intramammary infections (IMI) that limit profitable dairying. The course of infection is often associated with factors both related to the host and the bacterium. Aims of this study were to select S. aureus isolates from bovine IMI with different genotypic profiles harboring genes involved in adherence and biofilm production, to determine the behavior of these strains in contact with bovine mammary epithelial cells (MAC-T) and the expression of those genes during bacterial-cell early interactions. The genetic diversity of 20 S. aureus strains that were isolated from milk samples taken from cows with persistent-P and non-persistent-NP IMI was high, discriminated into 13 fingerprint groups. The occurrence of genes coding for S. aureus surface proteins (clfA, clfB, fnbA, fnbB, fib, cna) and biofilm formation (icaA, icaD, icaC, bap) and in vitro biofilm-forming ability was not related to strain clinical origin (NP or P). Internalization of S. aureus into MAC-T cells was strain-dependent and internalized bacteria overexpressed adherence and biofilm-forming genes compared with those that remained in the supernatant of co-cultures; particularly those genes encoding FnBPs and IcaD. Strains yielding highest invasion percentages were those able to overexpress fnBP, irrespectively of the presence of other evaluated genes. Strains from NP IMI showed a greater multiplication capacity in vitro compared with strains from P IMI. These results provide new insights about S. aureus differential gene expression of adhesion-internalization factors during early interaction with mammary epithelial cells.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceVeterinary microbiology 183 : 69-77. (2016)eng
dc.subjectStaphylococcus aureus
dc.subjectGenética
dc.subjectGeneticseng
dc.subjectMastitis
dc.subjectAnimal Diseaseseng
dc.subjectEnfermedades de los Animales
dc.titleDetection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenEEA Rafaela
dc.gic576
dc.description.filFil: Pereyra, Elizabet Amanda Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
dc.description.filFil: Renna, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
dc.description.filFil: Picech, Florencia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Biología Molecular e Inmunología Aplicadas; Argentina
dc.description.filFil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
dc.description.filFil: Andreotti, Carolina S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
dc.description.filFil: Russi, Romina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Biología Molecular e Inmunología Aplicadas; Argentina
dc.description.filFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
dc.description.filFil: Diez, Cristina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Biología Molecular e Inmunología Aplicadas; Argentina
dc.description.filFil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Biología Celular y Molecular Aplicada; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
dc.subtypecientifico


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