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N-Glycosylation in piroplasmids : Diversity within simplicity

Abstract
N-glycosylation has remained mostly unexplored in Piroplasmida, an order of tick-transmitted pathogens of veterinary and medical relevance. Analysis of 11 piroplasmid genomes revealed three distinct scenarios regarding N-glycosylation: Babesia sensu stricto (s.s.) species add one or two N-acetylglucosamine (NAcGlc) molecules to proteins; Theileria equi and Cytauxzoon felis add (NAcGlc)2-mannose, while B. microti and Theileria s.s. synthesize [ver mas...]
N-glycosylation has remained mostly unexplored in Piroplasmida, an order of tick-transmitted pathogens of veterinary and medical relevance. Analysis of 11 piroplasmid genomes revealed three distinct scenarios regarding N-glycosylation: Babesia sensu stricto (s.s.) species add one or two N-acetylglucosamine (NAcGlc) molecules to proteins; Theileria equi and Cytauxzoon felis add (NAcGlc)2-mannose, while B. microti and Theileria s.s. synthesize dolichol-P-P-NAcGlc and dolichol-P-P-(NAcGlc)2 without subsequent transfer to proteins. All piroplasmids possess the gene complement needed for the synthesis of the N-glycosylation substrates, dolichol-P and sugar nucleotides. The oligosaccharyl transferase of Babesia species, T. equi and C. felis, is predicted to be composed of only two subunits, STT3 and Ost1. Occurrence of short N-glycans in B. bovis merozoites was experimentally demonstrated by fluorescence microscopy using a NAcGlc-specific lectin. In vitro growth of B. bovis was significantly impaired by tunicamycin, an inhibitor of N-glycosylation, indicating a relevant role for N-glycosylation in this pathogen. Finally, genes coding for N-glycosylation enzymes and substrate biosynthesis are transcribed in B. bovis blood and tick stages, suggesting that this pathway is biologically relevant throughout the parasite life cycle. Elucidation of the role/s exerted by N-glycans will increase our understanding of these successful parasites, for which improved control measures are needed. [Cerrar]
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Author
Florin-Christensen, Mónica;   Rodriguez, Anabel Elisa;   Suarez, Carlos Esteban;   Ueti, Massaro W.;   Delgado, Fernando Oscar;   Echaide, Ignacio Eduardo;   Schnittger, Leonhard;  
Fuente
Pathogens 10 (1) : 50 (Enero 2021)
Date
2021-01
Editorial
MDPI
ISSN
2076-0817
URI
http://hdl.handle.net/20.500.12123/9115
https://www.mdpi.com/2076-0817/10/1/50
DOI
https://doi.org/10.3390/pathogens10010050
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Palabras Claves
Piroplasmea; Babesia; Theileria; Cytauxzoon; Glycosides; Glicosidos;
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