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An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle
Campero, Lucía María; Moreno-Gonzalo, Javier; Venturini, María Cecilia; Moré, Gastón; Dellarupe, Andrea; Rambeaud, Magdalena; Echaide, Ignacio Eduardo; Valentini, Beatriz Susana; Campero, Carlos Manuel; Moore, Prando Dadin; Cano, Dora Beatriz; Fort, Marcelo Cristian; Mota, Rinaldo A.; Serrano-Martínez, Marcos E.; Cruz-Vázquez, Carlos; Ortega-Mora, Luis M.; Álvarez-García, Gema (2018-01)We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories ... -
Babesia bovis biological clones and the inter-strain allelic diversity of the Bv80 gene support subpopulation selection as a mechanism involved in the attenuation of two virulent isolates
Baravalle, María Eugenia; Thompson, Carolina Soledad; Valentini, Beatriz Susana; Ferreira, Mariano B.; Torioni, Susana Marta; Florin-Christensen, Mónica; Echaide, Ignacio Eduardo (Elsevier, 2012-12-21)The virulence phenotype of Babesia bovis subpopulations was evaluated using biological clones derived from the high-virulence BboS2P and the low-virulence BboR1A strain and two original virulent isolates, BboL15 and BboL17, ... -
Babesiosis prevalence in malaria-endemic regions of Colombia
González Obando, Juliana; Echaide, Ignacio Eduardo; Pabón Vidal, Adriana Lucía; Piñeros Jiménez, Juan Gabriel; Blair Trujillo, Silvia; Tobón Castaño, Alberto (Wolters Kluwer, 2018-07)Background & objectives: The presence of Babesia spp in humans, bovine cattle and ticks (the transmitting vector) has not been well characterized in Colombia. Babesia infection in humans can be overlooked due to similarity ... -
Breed variability in the cellular mediated immune response to experimental Neospora caninum infection in heifers
Fiorani, Franco; Dallard, Bibiana; Cheuquepán Valenzuela, Felipe Andres; Sosa, Emiliano; Pardo, Alan Maxs; Gual, Ignacio; Morrell, Eleonora Lidia; Marin, Maia Solange; Quintana, Silvina; Canton, German Jose; Valentini, Beatriz Susana; Echaide, Ignacio Eduardo; Torioni, Susana Marta; Cobo, Eduardo Rubén; Corva, Pablo; Moore, Prando Dadin (Elsevier, 2024-09)Protozoan parasite Neospora caninum causes abortion in infected cattle while others remain asymptomatic. Host immunity plays a critical role in the outcome of bovine neosporosis. Despite extensive research, there is a ... -
Casos de anaplasmosis, babesiosis y tripanosomiasis bovina registradas en el INTA Rafaela (2012-2019)
Mazzucco Panizza, Matilde Nahimé; Novoa, María Belén; Sarli, Macarena; Signorini Porchiett, Marcelo Lisandro; Echaide, Ignacio Eduardo (Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste, 2022-06)Anaplasmosis, babesiosis y tripanosomiasis de los bovinos son enfermedades de importancia económica provocadas por Anaplasma marginale, Babesia bovis y/o Babesia bigemina, y Trypanosoma vivax, respectivamente. En este ... -
Characterization of GASA-1, a new vaccine candidate antigen of Babesia bovis
Flores, Daniela; Rodriguez, Anabel Elisa; Tomazic, Mariela Luján; Torioni, Susana Marta; Echaide, Ignacio Eduardo; Zamorano, Patricia Ines; Langellotti, Cecilia Ana; Araujo, Flabio Ribeiro de; Rolls, Peter; Schnittger, Leonhard; Florin-Christensen, Mónica (Elsevier, 2020-11)Surface proteins bound to the cell membrane by glycosylphosphatidylinositol (GPI) anchors are considered essential for the survival of pathogenic protozoans. In the case of the tick-transmitted hemoparasite Babesia bovis, ... -
Comparative transcriptome analysis of geographically distinct virulent and attenuated Babesia bovis strains reveals similar gene expression changes through attenuation
Pedroni, Monica J.; Sondgeroth, Kerry S.; Gallego-Lopez, Gina M.; Echaide, Ignacio Eduardo; Lau, Audrey OT (2013-11)Background: Loss of virulence is a phenotypic adaptation commonly seen in prokaryotic and eukaryotic pathogens. This mechanism is not well studied, especially in organisms with multiple host and life cycle stages such as ... -
Development and evaluation of a double-antigen sandwich ELISA to identify Anaplasma marginale–infected and A. centrale–vaccinated cattle
Sarli, Macarena; Thompson, Carolina Soledad; Novoa, María Belen; Valentini, Beatriz Susana; Mastropaolo, Mariano; Echaide, Ignacio Eduardo; Torioni, Susana Marta; Primo, María Evangelina (American Association of Veterinary Laboratory Diagnosticians, 2019-11)Bovine anaplasmosis is a worldwide infectious disease caused by the intraerythrocytic bacterium Anaplasma marginale, which is transmitted by ticks and fomites. A. centrale is a less virulent subspecies used as a live ... -
Estudio prospectivo de las primo infecciones por Babesia bovis en terneros Brahman y Brangus de un área enzoótica de Corrientes, Argentina
Martinez, I.; Jacobo, Roberto; Cipolini, M.F.; Martínez, Diana; Storani, C.A.; Ragazzi, A.; Echaide, Ignacio Eduardo; Torioni, Susana Marta (2014)Se evaluaron primo-infecciones causadas por Babesia bovis en terneros Brahman y Brangus en zona endémica de Corrientes, mediante la detección de: anticuerpos por ELISA-i, hematocrito y parasitemia en 48 vacas ... -
Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle
Novoa, María Belén; Valentini, Beatriz Susana; Torioni, Susana Marta; Primo, María Evangelina; Echaide, Ignacio Eduardo (American Association of Veterinary Laboratory Diagnosticians (AAVLD), 2020-05)Neospora caninum is a protozoan parasite that causes abortion and important economic losses in cattle worldwide. There are no treatments or vaccines available; disease control is based on diagnosis and herd management ... -
Evidence for extensive genetic diversity and substructuring of the Babesia bovis metapopulation
Flores, Daniela; Minichiello, Y.; Araujo, Flabio Ribeiro de; Shkap, V.; Benitez, Daniel Francisco; Echaide, Ignacio Eduardo; Rolls, P.; Mosqueda, J.; Pacheco, Maria Gabriela; Petterson, Maria Elizabeth; Florin-Christensen, Mónica; Schnittger, Leonhard (2013-11)Babesia bovis is a tick-transmitted haemoprotozoan and a causative agent of bovine babesiosis, a cattle disease that causes significant economic loss in tropical and subtropical regions. A panel of nineteen micro- and ... -
Experimental inoculation of Neospora caninum in pregnant water buffalo
Konrad, Jose Luis; Moore, Prando Dadin; Crudeli, Gustavo Angel; Caspe, Sergio Gaston; Cano, Dora Beatriz; Leunda, Maria Rosa; Lischinsky, Lilian Haydee; Regidor-Cerrillo, Javier; Odeon, Anselmo Carlos; Ortega-Mora, Luis M.; Echaide, Ignacio Eduardo; Campero, Carlos Manuel (Elsevier, 2012-06-08)The aim of this study was to characterize the pathogenesis of Neospora caninum in experimentally inoculated pregnant water buffalo (Bubalus bubalis). Twelve Mediterranean female water buffaloes ranging in age from 4 to 14 ... -
Immune response to Neospora caninum live tachyzoites in prepubertal female calves
Hecker, Yanina; Regidor-Cerrillo, Javier; Fiorani, Franco; Horcajo, Pilar; Soria, Ivana; Gual, Ignacio; Torioni, Susana Marta; Campero, Lucia; Echaide, Ignacio Eduardo; Alvarez-García, Gema; Ortega-Mora, Luis; Zamorano, Patricia Ines; Venturini, María Clara; Odeon, Anselmo Carlos; Canton, German Jose; Moore, Prando Dadin (Springer Berlin, 2019-09-04)The aim of the present study was to characterize the specific immune response in prepubertal female calves inoculated with Neospora caninum. Forty-eight N. caninum-seronegative 6-month-old Angus female calves were randomly ... -
Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection
Jaramillo Ortiz, Jose Manuel; Paoletta, Martina; Gravisaco, María José; Lopez Arias, Ludmila Sol; Montenegro, Valeria Noely; De La Fourniere, Sofia; Valenzano, Magali Nicole; Guillemi, Eliana Carolina; Valentini, Beatriz Susana; Echaide, Ignacio Eduardo; Farber, Marisa Diana; Wilkowsky, Silvina Elizabeth (Elsevier, 2019-10)Protection against the intraerythrocytic protozoan parasite Babesia bovis depends on both strong innate and adaptive immune response, this latter involving the presentation of parasite antigens to CD4+ T-lymphocytes by ... -
Mitigated clinical disease in water buffaloes experimentally infected with Babesia bovis
Benitez, Daniel Francisco; Mesplet, Maria; Echaide, Ignacio Eduardo; Torioni, Susana Marta; Schnittger, Leonhard; Florin-Christensen, Mónica (2018-07)Water buffaloes (Bubalus bubalis) are raised in tropical and subtropical regions of the world, and act as hosts of Babesia bovis parasites and the tick vector Rhipicephalus microplus. As no clinical cases of B. bovis-infection ... -
N-Glycosylation in piroplasmids : Diversity within simplicity
Florin-Christensen, Mónica; Rodriguez, Anabel Elisa; Suarez, Carlos Esteban; Ueti, Massaro W.; Delgado, Fernando Oscar; Echaide, Ignacio Eduardo; Schnittger, Leonhard (MDPI, 2021-01)N-glycosylation has remained mostly unexplored in Piroplasmida, an order of tick-transmitted pathogens of veterinary and medical relevance. Analysis of 11 piroplasmid genomes revealed three distinct scenarios regarding ... -
Neosporosis bovina en Argentina: a 25 años del primer reporte en el país
Campero, Lucía María; Moore, Prando Dadin; Echaide, Ignacio Eduardo; Campero, Carlos Manuel; Venturini, María Cecilia (Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 2021)La neosporosis es una enfermedad de gran impacto en el ganado vacuno debido a que causa abortos en el segundo y último trimestre de la gestación. En esta revisión se resume y discute la información sobre la misma, recopilada ... -
Neosporosis in Argentina: Past, present and future perspectives
Campero, Lucia Maria; Basso, W.; More, Gastón; Fiorani, Franco; Hecker, Yanina; Echaide, Ignacio Eduardo; Canton, German Jose; Cirone, Karina Mariela; Campero, Carlos Manuel; Venturini, M.C.; Moore, Prando Dadin (Elsevier, 2023-06)Neosporosis, caused by the protozoan Neospora caninum, was first diagnosed in Argentinean cattle in the 90's. With a national bovine stock of approximately 53 million head, the cattle industry is socially and economically ... -
Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows
Gual, Ignacio; Campero, Lucia Maria; Hecker, Yanina; Regidor-Cerrillo, Javier; Leunda, Maria Rosa; Odeon, Anselmo Carlos; Campero, Carlos Manuel; Torioni, Susana Marta; Echaide, Ignacio Eduardo; Estein, Silvia; Ortega Mora, Luis Miguel; Moore, Prando Dadin (Frontiers Media, 2022-06)The aim of this longitudinal study was to characterize the parasitemia of Neospora caninum and the associated immunological parameters in naturally infected beef cows for 10 months. The following groups were established: ... -
Relevamiento sanitario en bovinos de una comunidad indígena de la provincia del Neuquén, Argentina = Health survey in cattle from an indigenous community from Neuquén province, Argentina
Robles, Carlos Alejandro; Fernandez, C.; Parreño, Gladys; Scodellari, G.; Echaide, Ignacio Eduardo; Cabrera, Francisco Ra; Chodilef, Maria Marta (2015-02)Debido al escaso conocimiento que se posee sobre el estado sanitario del ganado perteneciente a comunidades indígenas de la Patagonia, se realizó un estudio que involucró a 47 crianceros de la Comunidad Millaín Currical, ...