Show simple item record

resumen

Abstract
PhoP is part of the two-component PhoPR system that regulates the expression of virulence genes of Mycobacteria. The goal of this work was to elucidate the role of PhoP in the mechanism that Mycobacterium bovis, the causative agent of bovine tuberculosis, displays upon stress. An analysis of gene expression and acidic growth curves indicated that M. bovis neutralized the external acidic environment by inducing and secreting ammonia. We found that PhoP is [ver mas...]
dc.contributor.authorGarcia, Elizabeth Andrea
dc.contributor.authorBlanco, Federico Carlos
dc.contributor.authorKlepp, Laura Ines
dc.contributor.authorPazos, Adriana Alejandra
dc.contributor.authorMcNeil, Michael
dc.contributor.authorJackson, Mary
dc.contributor.authorBigi, Fabiana
dc.date.accessioned2021-04-14T16:33:03Z
dc.date.available2021-04-14T16:33:03Z
dc.date.issued2021-02
dc.identifier.issn1878-1667
dc.identifier.otherhttps://doi.org/10.1016/j.cimid.2020.101593
dc.identifier.urihttp://hdl.handle.net/20.500.12123/9089
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S014795712030182X
dc.description.abstractPhoP is part of the two-component PhoPR system that regulates the expression of virulence genes of Mycobacteria. The goal of this work was to elucidate the role of PhoP in the mechanism that Mycobacterium bovis, the causative agent of bovine tuberculosis, displays upon stress. An analysis of gene expression and acidic growth curves indicated that M. bovis neutralized the external acidic environment by inducing and secreting ammonia. We found that PhoP is essential for ammonia production/secretion and its role in this process seems to be the induction of asparaginase and urease expression. We also demonstrated that the lack of PhoP negatively affected the synthesis of phthiocerol dimycocerosates. This finding is consistent with the role of the lipid anabolism in maintaining the redox environment upon stress in mycobacteria. Altogether the results of this study indicate that PhoP plays an important role in the response mechanisms to stress of M. bovis.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud”es_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceComparative Immunology, Microbiology and Infectious Diseases 74 : 101593 (Febrero 2021)es_AR
dc.subjectMycobacterium bovises_AR
dc.subjectStresseng
dc.subjectEstreses_AR
dc.subjectVirulenceeng
dc.subjectVirulenciaes_AR
dc.subjectMycobacterium bovis Infectionseng
dc.subjectInfeccion por Mycobacterium bovises_AR
dc.subjectTuberculosiseng
dc.subjectAmmoniaeng
dc.subjectAmoníacoes_AR
dc.titleRole of PhoPR in the response to stress of Mycobacterium bovises_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Pazos, Adriana Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentinaes_AR
dc.description.filFil: McNeil, Michael. Colorado State University. Department of Microbiology, Immunology and Pathology; Estados Unidoses_AR
dc.description.filFil: Jackson, Mary. Colorado State University. Department of Microbiology, Immunology and Pathology; Estados Unidoses_AR
dc.description.filFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentinaes_AR
dc.description.filFil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record