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Abstract
Differential epigenetic (DNA cytosine methylation) and gene expression patterns were investigated in reproductive and vegetative organs from Ilex paraguariensis and I. dumosa, at distinct developmental stages. We aimed at contributing towards elucidating major molecular changes underlying the sexual differentiation processes which, in these dioecious species, are completely unknown. Simultaneously, as a first step towards the development of an early [ver mas...]
dc.contributor.authorCascales, Jimena
dc.contributor.authorAcevedo, Raúl Maximiliano
dc.contributor.authorPaiva, Daniela Ivana
dc.contributor.authorGottlieb, Alexandra Marina
dc.date.accessioned2021-03-31T13:06:46Z
dc.date.available2021-03-31T13:06:46Z
dc.date.issued2021-03
dc.identifier.issn0918-9440
dc.identifier.issn1618-0860
dc.identifier.otherhttps://doi.org/10.1007/s10265-021-01279-3
dc.identifier.urihttp://hdl.handle.net/20.500.12123/9008
dc.identifier.urihttps://link.springer.com/article/10.1007/s10265-021-01279-3
dc.description.abstractDifferential epigenetic (DNA cytosine methylation) and gene expression patterns were investigated in reproductive and vegetative organs from Ilex paraguariensis and I. dumosa, at distinct developmental stages. We aimed at contributing towards elucidating major molecular changes underlying the sexual differentiation processes which, in these dioecious species, are completely unknown. Simultaneously, as a first step towards the development of an early sexing system, we searched for promising molecular markers. This was assessed through Methylation Sensitive Amplified Polymorphism (MSAP) and Amplified Fragment Length Polymorphism on cDNA (cDNA-AFLP) techniques, applying discriminant multivariate analyses, and bioinformatic characterization of differential fragments. A significant positive correlation was found between epigenetic and indirect ‘genetic’ information for both species, indicating influence of the genetic background on the epigenetic variation. Higher epigenetic than genetic diversities were estimated. Our outcomes showed up to 1.86 times more representation of mCG subepiloci than mCCG in all organs sampled. Along the maturing stages of floral buds, the frequency of mCG evidenced an incremental trend, whereas mCCG and unmethylated conditions showed opposite tendencies. Reproductive and vegetative samples tended to cluster apart based on epigenetic patterns; at gene expression level, organs exhibited clear-cut distinctive patterns, nonetheless profiles of young leaves and floral primordia resemble. Epigenetic and expression data allowed discrimination of I. dumosa´s samples according to the gender of the donor; more elusive patterns were observed for I. paraguariensis. In total, 102 differentially methylated and expressed fragments were characterized bioinformatically. Forty-three were annotated in various functional categories; four candidate markers were validated through qPCR, finding statistical differences among organs but not among sexes. The methylation condition of epilocus C13m33 appears as indicative of gender in both species. Thirty-three organ-specific and 34 gender-specific methylated markers were discriminated and deserve further research, particularly those expressed in leaves. Our study contributes concrete candidate markers with potential for practical application.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceJournal of Plant Research ( Published: 23 March 2021)es_AR
dc.subjectIlexes_AR
dc.subjectIlex paraguariensises_AR
dc.subjectExpresión Génicaes_AR
dc.subjectGene Expressioneng
dc.subjectMarcadores Genéticoses_AR
dc.subjectGenetic Markerseng
dc.subjectAnálisis Multivariantees_AR
dc.subjectMultivariate Analysiseng
dc.subjectMetilaciónes_AR
dc.subjectMethylationeng
dc.subject.otherMarcadores Moleculareses_AR
dc.subject.otherYerba Matees_AR
dc.titleDifferential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex specieses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Montecarloes_AR
dc.description.filFil: Cascales, Jimena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentinaes_AR
dc.description.filFil: Cascales, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Acevedo, Raúl M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentinaes_AR
dc.description.filFil: Acevedo, Raúl M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentinaes_AR
dc.description.filFil: Paiva, Daniela Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentinaes_AR
dc.description.filFil: Paiva, Daniela Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentinaes_AR
dc.description.filFil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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