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Abstract
Snakin‐1 (SN1), a cysteine‐rich peptide with broad‐spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highly evolutionary conservation with 91–98% identity values. Potato plants (S. tuberosum subsp. tuberosum cv. Kennebec) were transformed via Agrobacterium [ver mas...]
dc.contributor.authorAlmasia, Natalia Ines
dc.contributor.authorBazzini, Ariel Alejandro
dc.contributor.authorHopp, Horacio Esteban
dc.contributor.authorVazquez Rovere, Cecilia
dc.date.accessioned2021-01-27T13:23:19Z
dc.date.available2021-01-27T13:23:19Z
dc.date.issued2008-05
dc.identifier.issn1364-3703
dc.identifier.otherhttps://doi.org/10.1111/j.1364-3703.2008.00469.x
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8655
dc.identifier.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640289/
dc.description.abstractSnakin‐1 (SN1), a cysteine‐rich peptide with broad‐spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highly evolutionary conservation with 91–98% identity values. Potato plants (S. tuberosum subsp. tuberosum cv. Kennebec) were transformed via Agrobacterium tumefaciens with a construct encoding the S. chacoense SN1 gene under the regulation of the ubiquitous CaMV 35S promoter. Transgenic lines were molecularly characterized and challenged with either Rhizoctonia solani or Erwinia carotovora to analyse whether constitutive in vivo overexpression of the SN1 gene may lead to disease resistance. Only transgenic lines that accumulated high levels of SN1 mRNA exhibited significant symptom reductions of R. solani infection such as stem cankers and damping‐off. Furthermore, these overexpressing lines showed significantly higher survival rates throughout the fungal resistance bioassays. In addition, the same lines showed significant protection against E. carotovora measured as: a reduction of lesion areas (from 46.5 to 88.1% with respect to the wild‐type), number of fallen leaves and thickened or necrotic stems. Enhanced resistance to these two important potato pathogens suggests in vivo antifungal and antibacterial activity of SN1 and thus its possible biotechnological application.es_AR
dc.formatapplication/pdfeng
dc.language.isoenges_AR
dc.publisherWiley-Blackwelleng
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.sourceMolecular plant pathology 9 (3) : 329-338. (Mayo 2008)es_AR
dc.subjectDisease Resistanceeng
dc.subjectResistencia a la Enfermedades_AR
dc.subjectRhizoctonia Solanies_AR
dc.subjectPectobacterium Carotovorumes_AR
dc.subjectTransgenic Plantseng
dc.subjectPlantas Transgénicases_AR
dc.subjectPotatoeseng
dc.subjectPapaes_AR
dc.subjectSolanumes_AR
dc.subject.otherErwinia Carotovoraes_AR
dc.titleOverexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plantseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.es_AR
dc.description.filFil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.es_AR
dc.description.filFil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Almasia, Natalia Ines. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Área de Biotecnología; Argentina
dc.description.filFil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Área de Biotecnología; Argentina
dc.subtypecientifico


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