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Abstract
Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was isolated from the potato genome and sequenced. Bioinformatics analyses revealed a total of 55 potential regulatory motifs related to tissue-specificity, [ver mas...]
dc.contributor.authorAlmasia, Natalia Ines
dc.contributor.authorNahirñak, Vanesa
dc.contributor.authorHopp, Horacio Esteban
dc.contributor.authorVazquez Rovere, Cecilia
dc.date.accessioned2021-01-22T18:01:10Z
dc.date.available2021-01-22T18:01:10Z
dc.date.issued2010-09
dc.identifier.issn0717-3458
dc.identifier.other10.2225/vol13-issue5-fulltext-12
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8640
dc.identifier.urihttps://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-34582010000500008&lng=es&nrm=iso
dc.description.abstractSnakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was isolated from the potato genome and sequenced. Bioinformatics analyses revealed a total of 55 potential regulatory motifs related to tissue-specificity, stress, defence and hormone responsiveness, among others. PStSN1 spatial and temporal activity was studied in transgenic Arabidopsis plants expressing a reporter gene under this promoter control (PStSN1::GUS). Histochemical staining revealed PStSN1::GUS expression in the root vasculature, cotyledons, young leaves and floral organs. Moreover, GUS staining was detected in young developmental stages gradually decreasing as the plant aged. Stress treatments on transgenic plants showed that PStSN1 activity was induced by high/low temperature and wounding. The characterization of PStSN1 in a model plant establishes a framework for the understanding of its possible biological functions and provides a potential tool for plant modification through genetic engineering.es_AR
dc.formatapplication/pdfeng
dc.language.isoenges_AR
dc.publisherPontificia Universidad Catolica de Valparaisoes_AR
dc.rightsinfo:eu-repo/semantics/openAccesseng
dc.sourceElectronic journal of biotechnology 13 (5) : 1-21. (Septiembre 2010)es_AR
dc.subjectPeptideseng
dc.subjectPéptidoses_AR
dc.subjectAntimicrobialseng
dc.subjectAntimicrobianoses_AR
dc.subjectPotatoeseng
dc.subjectPapaes_AR
dc.subjectSolanum Tuberosumes_AR
dc.subjectGenetic Isolationeng
dc.subjectAislamiento Genéticoes_AR
dc.subjectTemperatureeng
dc.subjectTemperaturaes_AR
dc.subjectTransgenic Plantseng
dc.subjectPlantas Transgénicases_AR
dc.titleIsolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and woundingeng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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