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Abstract
The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures [ver mas...]
dc.contributor.authorCarneiro, Paulo A.M.
dc.contributor.authorPasquatti, Taynara N.
dc.contributor.authorTakatani, Haruo
dc.contributor.authorZumarraga, Martin Jose
dc.contributor.authorMarfil, Maria Jimena
dc.contributor.authorBarnard, Christian
dc.contributor.authorFitzgerald, Scott D.
dc.contributor.authorAbramovitch, Robert B.
dc.contributor.authorAraujo, Flabio Ribeiro de
dc.contributor.authorKaneene, John B.
dc.date.accessioned2021-01-05T17:58:19Z
dc.date.available2021-01-05T17:58:19Z
dc.date.issued2020-02
dc.identifier.issn2053-1095
dc.identifier.otherhttps://doi.org/10.1002/vms3.203
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8553
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1002/vms3.203
dc.description.abstractThe aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherWileyes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceVeterinary Medicine and Science 6 (1) : 133-141 (Febrero 2020)es_AR
dc.subjectMycobacterium bovises_AR
dc.subjectTuberculosises_AR
dc.subjectCattleeng
dc.subjectGanado Bovinoes_AR
dc.subjectBuffalo Breedseng
dc.subjectRazas de Búfaloes_AR
dc.subjectGenotypeseng
dc.subjectGenotiposes_AR
dc.subjectBrasiles_AR
dc.titleMolecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Braziles_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidoses_AR
dc.description.filFil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasiles_AR
dc.description.filFil: Takatani, Haruo. Agencia de Defesa Agropecuaria do Amazonas; Brasiles_AR
dc.description.filFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Barnard, Christian. Agencia de Defesa Agropecuaria do Amazonas; Brasiles_AR
dc.description.filFil: Fitzgerald, Scott D. Michigan State University. Veterinary Diagnostic Laboratory; Estados Unidoses_AR
dc.description.filFil: Abramovitch, Robert B. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidoses_AR
dc.description.filFil: Araujo, Flabio Ribeiro de. Centro Nacional de Pesquisa de Gado de Corte; Brasiles_AR
dc.description.filFil: Kaneene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidoses_AR
dc.subtypecientifico


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