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Resumen
Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we evaluated a strategy to detect M. bovis–infected herds by Touch-Down IS6110 polymerase chain reaction (PCR) in bulk tank raw milk from dairy farms. We evaluated 177 samples from herds with the official tuberculosis free certificate (TFC) [ver mas...]
dc.contributor.authorZumarraga, Martin Jose
dc.contributor.authorSoutullo, Adriana
dc.contributor.authorGarcia, Maria Ines
dc.contributor.authorMarini, M. Rocío
dc.contributor.authorAbdala, Alejandro Ariel
dc.contributor.authorTarabla, Hector Dante
dc.contributor.authorEchaide, Susana T. de
dc.contributor.authorLopez, Marcela
dc.contributor.authorZervini, Elsa
dc.contributor.authorCanal, Ana María
dc.contributor.authorCataldi, Angel Adrian
dc.date.accessioned2020-11-24T16:44:41Z
dc.date.available2020-11-24T16:44:41Z
dc.date.issued2012-02
dc.identifier.issn1556-7125
dc.identifier.otherhttp://doi.org/10.1089/fpd.2011.0963
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8317
dc.identifier.urihttps://www.liebertpub.com/doi/10.1089/fpd.2011.0963
dc.description.abstractBovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we evaluated a strategy to detect M. bovis–infected herds by Touch-Down IS6110 polymerase chain reaction (PCR) in bulk tank raw milk from dairy farms. We evaluated 177 samples from herds with the official tuberculosis free certificate (TFC) and 80 from herds without the certificate, non-tuberculosis-free certificate (NTFC), from 10 departments of Santa Fe province, Argentina. To avoid the effect of Taq polymerase inhibitors, a dilution of DNA template was performed. Positive PCR results were obtained in 102 (40%) of the samples, whereas negative ones were obtained in 155 (60%) of the samples. Importantly, 44% of NTFC and 38% of TFC samples were positive. All samples were subjected to culture in Löwenstein Jensen and Stonebrink media with no positive isolation. The negative predictive value (NPV) of PCR in the TFC group was 95%, while the positive predictive value (PPV) of PCR in the NTFC group was 51%. Based on these results, this work proposes a method that should be applied regularly to detect M. bovis--infected dairy herds, complementary to the official test of tuberculin, or purifed protein derivative (PPD), to control dairy herds, especially those free of tuberculosis.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherMary Ann Liebert Inc.es_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceFoodborne Pathogens and Disease 9 (2) : 132-137 (Febrero 2012)es_AR
dc.subjectMycobacterium bovises_AR
dc.subjectDairy Herdseng
dc.subjectRebaño de Lechees_AR
dc.subjectPCRes_AR
dc.subjectMilkeng
dc.subjectLechees_AR
dc.subjectTuberculosises_AR
dc.subjectArgentinaes_AR
dc.titleDetection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk sampleses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Soutullo, Adriana. Ministerio de la Producción. Dirección General de Sanidad Animal. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentinaes_AR
dc.description.filFil: Garcia, Maria Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Inmunología Básica; Argentinaes_AR
dc.description.filFil: Marini, M. Rocío. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Básica; Argentinaes_AR
dc.description.filFil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Echaide, Susana T. de. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Lopez, Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentinaes_AR
dc.description.filFil: Zervini, Elsa. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentinaes_AR
dc.description.filFil: Canal, Ana María. Ministerio de la Producción. Dirección General de Sanidad Animal. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Básica; Argentinaes_AR
dc.description.filFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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