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In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue
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dc.contributor.author | Trangoni, Marcos David | |
dc.contributor.author | Gioffre, Andrea | |
dc.contributor.author | Ceron Cucchi, Maria Esperanza | |
dc.contributor.author | Caimi, Karina Cynthia | |
dc.contributor.author | Ruybal, Paula | |
dc.contributor.author | Zumarraga, Martin Jose | |
dc.contributor.author | Cravero, Silvio Lorenzo Pedro | |
dc.date.accessioned | 2020-11-10T16:35:00Z | |
dc.date.available | 2020-11-10T16:35:00Z | |
dc.date.issued | 2015-06 | |
dc.identifier.issn | 1678-4405 | |
dc.identifier.other | https://doi.org/10.1590/S1517-838246220131206 | |
dc.identifier.uri | https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200619&lng=en&nrm=iso&tlng=en | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/8234 | |
dc.description.abstract | In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue and SYBR GreenTM allowed direct naked-eye detection with identical sensitivity as agarose gel electrophoresis. We included the LAMP-based protocol in a rapid identification scheme of the respective pathogens, and all tested isolates were correctly identified within 2 to 3 h. In addition, both protocols were suitable for specifically identifying the respective pathogens; in the case of Brucella, it also allowed the identification of all the biovars tested. We conclude that LAMP is a suitable rapid molecular typing tool that could help to shorten the time required to identify insidious bacteria in low-complexity laboratories, mainly in developing countries. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Sociedade Brasileira de Microbiologia | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/AERG-232121/AR./Desarrollo de métodos de diagnóstico molecular aplicados a la detección y tipificación de microorganismos patógenos | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | Brazilian Journal of Microbiology 46 (2) : 619-626 (Junio 2015) | es_AR |
dc.subject | Isothermal Processes | eng |
dc.subject | Procesos Isotermales | es_AR |
dc.subject | Molecular Biology | eng |
dc.subject | Biología Molecular | es_AR |
dc.subject | Brucellosis | eng |
dc.subject | Brucelosis | es_AR |
dc.subject | Paratuberculosis | es_AR |
dc.subject | Mycobacterium avium subsp. paratuberculosis | es_AR |
dc.title | LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Ceron Cucchi, Maria Esperanza. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina | es_AR |
dc.description.fil | Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Cravero, Silvio Lorenzo Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.subtype | cientifico |
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