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Abstract
There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and [ver mas...]
dc.contributor.authorMorcillo, Nora
dc.contributor.authorImperiale, Belen Rocio
dc.contributor.authorDi Giulio, Angela Beatríz
dc.contributor.authorZumarraga, Martin Jose
dc.contributor.authorTakif, Howard
dc.contributor.authorCataldi, Angel Adrian
dc.date.accessioned2020-11-04T17:10:05Z
dc.date.available2020-11-04T17:10:05Z
dc.date.issued2014-12
dc.identifier.issn1472-9792
dc.identifier.otherhttps://doi.org/10.1016/j.tube.2014.08.003
dc.identifier.urihttp://hdl.handle.net/20.500.12123/8191
dc.identifier.urihttps://www.sciencedirect.com/science/article/abs/pii/S1472979214203908
dc.description.abstractThere has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceTuberculosis 94 (6) : 672-677 (Diciembre 2014)es_AR
dc.subjectDrug Resistanceeng
dc.subjectResistencia a Medicamentoses_AR
dc.subjectTuberculosises_AR
dc.subjectVigoureng
dc.subjectVigores_AR
dc.subjectHouseholdseng
dc.subjectHogareses_AR
dc.subjectMycobacterium tuberculosises_AR
dc.subject.otherFamily Transmissioneng
dc.subject.otherTransmisión Familiares_AR
dc.titleFitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contactses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentinaes_AR
dc.description.filFil: Imperiale, Belen Rocio. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentinaes_AR
dc.description.filFil: Di Giulio, Angela Beatríz. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; Argentinaes_AR
dc.description.filFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.description.filFil: Takif, Howard. Instituto Venezolano de Investigaciones Científicas; Venezuelaes_AR
dc.description.filFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentinaes_AR
dc.subtypecientifico


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