Show simple item record

resumen

Abstract
Brucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats analysis (MLVA) is a powerful tool to genotype Brucella spp. However, data regarding B. ovis genotyping is scarce. Thus, the aim of this study was to characterize the molecular [ver mas...]
dc.contributor.authorAlvarez, Lucía Paula
dc.contributor.authorRuiz-Villalobos, Nazaret
dc.contributor.authorSuárez-Esquivel, Marcela
dc.contributor.authorThomson, Nicholas R.
dc.contributor.authorMarcellino, Romanela
dc.contributor.authorVíquez-Ruiz, Eunice
dc.contributor.authorRobles, Carlos Alejandro
dc.contributor.authorGuzmán-Verri, Caterina
dc.date.accessioned2020-09-21T12:17:40Z
dc.date.available2020-09-21T12:17:40Z
dc.date.issued2020-06
dc.identifier.issn0378-1135
dc.identifier.otherhttps://doi.org/10.1016/j.vetmic.2020.108703
dc.identifier.urihttp://hdl.handle.net/20.500.12123/7928
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S037811351931452X
dc.description.abstractBrucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats analysis (MLVA) is a powerful tool to genotype Brucella spp. However, data regarding B. ovis genotyping is scarce. Thus, the aim of this study was to characterize the molecular diversity of B. ovis field-strains in Argentina. A total of 115 isolates of B. ovis from Argentina and Uruguay were genotyped using MLVA-16 and analyzed altogether with 14 publicly available B. ovis genotypes from Brazil. The Discriminatory Power (D) was 0.996 for MLVA-16 and 0.0998 for MLVA-8 and MLVA-11. Analysis of MLVA-16 revealed 100 different genotypes, all of them novel, including 90 unique ones. There was no correlation between geographical distribution and genotype and results showed a higher diversity within provinces than between provinces. Clustering analysis of the strains from Argentina, Uruguay and Brazil revealed that the 129 isolates were grouped into two clades. Whole Genome Sequencing analysis of the 19 B. ovis genomes available in public databases, and including some of the Argentinian strains used in this study, revealed clustering of the Argentinian isolates and closer relationship with B. ovis from New Zealand and Australia. This work adds new data to the poorly understood distribution map of genotypes regionally and worldwide for B. ovis and it constitutes the largest study of B. ovis molecular genotyping until now.es_AR
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVeterinary Microbiology 245 : Art. 108703 (June 2020)es_AR
dc.subjectBrucella Ovises_AR
dc.subjectBrucellaes_AR
dc.subjectBrucellosiseng
dc.subjectBrucelosises_AR
dc.subjectBrucella Melitensises_AR
dc.subjectGenotiposes_AR
dc.subjectGenotypeseng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectArgentina
dc.titleMolecular characterization of Brucella ovis in Argentinaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEstación Experimental Agropecuaria Barilochees_AR
dc.description.filFil: Alvarez, Lucía Paula. Universidad Nacional del Comahue. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentinaes_AR
dc.description.filFil: Ruiz-Villalobos, Nazaret. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Ricaes_AR
dc.description.filFil: Suárez-Esquivel, Marcela. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Ricaes_AR
dc.description.filFil: Thomson, Nicholas R. Wellcome Trust Sanger Institute. Parasites and Microbes from Pathogen Genomics; Reino Unidoes_AR
dc.description.filFil: Marcellino, Romanela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Sanidad Animal; Argentinaes_AR
dc.description.filFil: Víquez-Ruiz, Eunice. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Ricaes_AR
dc.description.filFil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area de Produccion Animal. Grupo de Sanidad Animal; Argentinaes_AR
dc.description.filFil: Guzmán-Verri, Caterina. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Ricaes_AR
dc.subtypecientifico


Files in this item

Thumbnail

This item appears in the following Collection(s)

common

Show simple item record