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Abstract
Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas [ver mas...]
dc.contributor.authorDarqui, Flavia Soledad
dc.contributor.authorHopp, Horacio Esteban
dc.contributor.authorLopez Bilbao, Marisa Gisela
dc.date.accessioned2020-09-16T17:47:50Z
dc.date.available2020-09-16T17:47:50Z
dc.date.issued2020-08
dc.identifier.issn2637-7659
dc.identifier.otherhttp://doi.org/10.31031/MCDA.2020.07.000656
dc.identifier.urihttp://hdl.handle.net/20.500.12123/7913
dc.identifier.urihttps://crimsonpublishers.com/mcda/fulltext/MCDA.000656.php
dc.description.abstractSpecific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. This review summarizes the main knowledge and experiences attained from the use of the ALS gene as a target in CRISPR/Cas studies.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherCrimson Publisherses_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceModern Concepts & Developments in Agronomy 7 (2) : 700-711 (Agosto 2020)es_AR
dc.subjectMutagenesiseng
dc.subjectMutagénesises_AR
dc.subjectAmino Acidseng
dc.subjectAminoácidoses_AR
dc.subjectResistance to Herbicideseng
dc.subjectResistencia a los Herbicidases_AR
dc.subjectGeneses_AR
dc.subject.otherAcetolactate Synthaseeng
dc.subject.otherAcetolactato Sintasaes_AR
dc.titleThe ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?es_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentinaes_AR
dc.description.filFil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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