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Resumen
Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana
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dc.contributor.author | Gomez, Evangelina Raquel | |
dc.contributor.author | Cassani, María Florencia | |
dc.contributor.author | Lucero, Maria Soledad | |
dc.contributor.author | Parreño, Viviana | |
dc.contributor.author | Chimeno Zoth, Silvina Andrea | |
dc.contributor.author | Berinstein, Analia | |
dc.date.accessioned | 2020-08-25T12:18:32Z | |
dc.date.available | 2020-08-25T12:18:32Z | |
dc.date.issued | 2020-05 | |
dc.identifier.issn | 2191-0855 | |
dc.identifier.other | https://doi.org/10.1186/s13568-020-01029-z | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/7766 | |
dc.identifier.uri | https://amb-express.springeropen.com/articles/10.1186/s13568-020-01029-z | |
dc.description.abstract | Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. The SVP, which were observed by electronic microscopy, proved to be antigenically and immunogenically similar to the virion. Indeed, anti-IBDV antibodies from samples of infected birds recognized these SVP and, when injected intramuscularly, these subviral particles also evoked a humoral immune response in chickens. We developed an in-house ELISA using SVP as coating reagent that demonstrated to be highly accurate and in good agreement with a commercial ELISA. This study demonstrates that the recombinant antigen generated and the technology used to produce it are suitable for developing a diagnostic tool against Infectious bursal disease. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | SpringerOpen | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | AMB Express 10 : article number: 95 (2020) | es_AR |
dc.subject | Enfermedades de los Animales | es_AR |
dc.subject | Animal Diseases | eng |
dc.subject | Virus Bursitis Infecciosa | es_AR |
dc.subject | Infectious Bursal Disease Virus | eng |
dc.subject | Diagnóstico | es_AR |
dc.subject | Diagnosis | eng |
dc.subject | Biorreactores | es_AR |
dc.subject | Bioreactors | eng |
dc.subject.other | Nicotiana benthamiana | es_AR |
dc.title | Development of diagnostic tools for IBDV detection using plants as bioreactors | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Gomez, Evangelina Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Cassani, María Florencia. Universidad Nacional de Luján; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Lucero, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | es_AR |
dc.description.fil | Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Berinstein, Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina | es_AR |
dc.subtype | cientifico |
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