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Resumen
In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic
potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite
species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal
was to assess the microbial community compositions and metabolic capacity, and to identify genes
involved in lignocellulose degradation. Individuals from both
[ver mas...]
dc.contributor.author | Romero Victorica, Matias | |
dc.contributor.author | Soria, Marcelo Abel | |
dc.contributor.author | Batista-García, Ramón Alberto | |
dc.contributor.author | Ceja-Navarro, Javier A. | |
dc.contributor.author | Vikram, Surendra | |
dc.contributor.author | Ortiz, Maximiliano | |
dc.contributor.author | Ontañon, Ornella | |
dc.contributor.author | Ghio, Silvina | |
dc.contributor.author | Martínez-Ávila, Liliana | |
dc.contributor.author | Quintero García, Omar Jasiel | |
dc.contributor.author | Etcheverry, Clara | |
dc.contributor.author | Campos, Eleonora | |
dc.contributor.author | Cowan, Donald | |
dc.contributor.author | Arneodo Larochette, Joel Demian | |
dc.contributor.author | Talia, Paola Mónica | |
dc.date.accessioned | 2020-03-16T11:04:38Z | |
dc.date.available | 2020-03-16T11:04:38Z | |
dc.date.issued | 2020-03-02 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | https://doi.org/10.1038/s41598-020-60850-5 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/6945 | |
dc.identifier.uri | https://www.nature.com/articles/s41598-020-60850-5 | |
dc.description.abstract | In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes. These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4- xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Springer Nature | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | Scientific Reports 10 : 3864 (2020) | es_AR |
dc.subject | Genomics | eng |
dc.subject | Nucleotide Sequence | eng |
dc.subject | Genómica | es_AR |
dc.subject | Secuencia Nucleotídica | es_AR |
dc.title | Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Microbiología y Zoología Agrícola (IMyZA) | es_AR |
dc.description.fil | Fil: Victorica, Matias Romero. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Batista-García, Ramón Alberto. Universidad Autónoma Estado Morelos; México | es_AR |
dc.description.fil | Fil: Ceja-Navarro, Javier A. Lawrence Berkeley National Laboratory. Biological Systems and Engineering Division ; Estados Unidos | es_AR |
dc.description.fil | Fil: Vikram, Surendra. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica | es_AR |
dc.description.fil | Fil: Ortiz, Maximiliano. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica | es_AR |
dc.description.fil | Fil: Ontañon, Ornella. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Martínez-Ávila, Liliana. Universidad Autónoma Estado Morelos; México | es_AR |
dc.description.fil | Fil: Quintero García, Omar Jasiel. Universidad Autónoma Estado Morelos; México | es_AR |
dc.description.fil | Fil: Etcheverry, Clara. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Biología de los Invertebrados; Argentina | es_AR |
dc.description.fil | Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Cowan, Donald. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica | es_AR |
dc.description.fil | Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
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