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Abstract
In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both [ver mas...]
dc.contributor.authorRomero Victorica, Matias
dc.contributor.authorSoria, Marcelo Abel
dc.contributor.authorBatista-García, Ramón Alberto
dc.contributor.authorCeja-Navarro, Javier A.
dc.contributor.authorVikram, Surendra
dc.contributor.authorOrtiz, Maximiliano
dc.contributor.authorOntañon, Ornella
dc.contributor.authorGhio, Silvina
dc.contributor.authorMartínez-Ávila, Liliana
dc.contributor.authorQuintero García, Omar Jasiel
dc.contributor.authorEtcheverry, Clara
dc.contributor.authorCampos, Eleonora
dc.contributor.authorCowan, Donald
dc.contributor.authorArneodo Larochette, Joel Demian
dc.contributor.authorTalia, Paola Mónica
dc.date.accessioned2020-03-16T11:04:38Z
dc.date.available2020-03-16T11:04:38Z
dc.date.issued2020-03-02
dc.identifier.issn2045-2322
dc.identifier.otherhttps://doi.org/10.1038/s41598-020-60850-5
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6945
dc.identifier.urihttps://www.nature.com/articles/s41598-020-60850-5
dc.description.abstractIn this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes. These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4- xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringer Naturees_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceScientific Reports 10 : 3864 (2020)es_AR
dc.subjectGenomicseng
dc.subjectNucleotide Sequenceeng
dc.subjectGenómicaes_AR
dc.subjectSecuencia Nucleotídicaes_AR
dc.titleNeotropical termite microbiomes as sources of novel plant cell wall degrading enzymeses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenInstituto de Microbiología y Zoología Agrícola (IMyZA)es_AR
dc.description.filFil: Victorica, Matias Romero. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Batista-García, Ramón Alberto. Universidad Autónoma Estado Morelos; Méxicoes_AR
dc.description.filFil: Ceja-Navarro, Javier A. Lawrence Berkeley National Laboratory. Biological Systems and Engineering Division ; Estados Unidoses_AR
dc.description.filFil: Vikram, Surendra. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfricaes_AR
dc.description.filFil: Ortiz, Maximiliano. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfricaes_AR
dc.description.filFil: Ontañon, Ornella. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Martínez-Ávila, Liliana. Universidad Autónoma Estado Morelos; Méxicoes_AR
dc.description.filFil: Quintero García, Omar Jasiel. Universidad Autónoma Estado Morelos; Méxicoes_AR
dc.description.filFil: Etcheverry, Clara. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Biología de los Invertebrados; Argentinaes_AR
dc.description.filFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Cowan, Donald. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfricaes_AR
dc.description.filFil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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