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Abstract
Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, [ver mas...]
dc.contributor.authorBigi, María Mercedes
dc.contributor.authorVazquez, Cristina Lourdes
dc.contributor.authorCastelão, Ana Beatriz C.
dc.contributor.authorGarcia, Elizabeth Andrea
dc.contributor.authorCataldi, Angel Adrian
dc.contributor.authorJackson, Mary
dc.contributor.authorMcNeil, Michael
dc.contributor.authorSoria, Marcelo Abel
dc.contributor.authorZumarraga, Martin Jose
dc.contributor.authorCabruja, Matias
dc.contributor.authorGago, Gabriela
dc.contributor.authorBlanco, Federico Carlos
dc.contributor.authorNishibe, Christiane
dc.contributor.authorAlmeida Junior, Nalvo Franco de
dc.contributor.authorAraujo, Flabio Ribeiro de
dc.contributor.authorBigi, Fabiana
dc.date.accessioned2020-01-15T12:59:14Z
dc.date.available2020-01-15T12:59:14Z
dc.date.issued2019-12
dc.identifier.issn0378-1135
dc.identifier.otherhttps://doi.org/10.1016/j.vetmic.2019.108482
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6681
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0378113519301099?via%3Dihub
dc.description.abstractMycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines.eng
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherElsevier
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceVeterinary microbiology 239 : 108482. (December 2019)es_AR
dc.subjectMycobacterium Bovises_AR
dc.subjectGenomeseng
dc.subjectGenomases_AR
dc.subjectVirulenceeng
dc.subjectVirulenciaes_AR
dc.subjectMutationeng
dc.subjectMutaciónes_AR
dc.subjectPathogenicityeng
dc.subjectPatogenicidades_AR
dc.subjectBovinaeeng
dc.subjectBovinaes_AR
dc.subjectTuberculosises_AR
dc.subjectMycobacterium Bovis Infectionseng
dc.subjectInfección por Mycobacterium Bovises_AR
dc.subjectVaccineseng
dc.subjectVacunaes_AR
dc.titleAnalysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profileseng
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Castelão, Ana Beatriz C. Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia; Brasiles_AR
dc.description.filFil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Jackson, Mary. Colorado State University. Dept. of Microbiology, Immunology and Pathology; Estados Unidoses_AR
dc.description.filFil: McNeil, Michael. Colorado State University. Dept. of Microbiology, Immunology and Pathology; Estados Unidoses_AR
dc.description.filFil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Cabruja, Matias. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentinaes_AR
dc.description.filFil: Gago, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentinaes_AR
dc.description.filFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Nishibe, Christiane. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; Brasiles_AR
dc.description.filFil: Almeida Junior, Nalvo Franco de. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; Brasiles_AR
dc.description.filFil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasiles_AR
dc.description.filFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de investigaciones Científicas y Tecnológicas; Argentinaes_AR
dc.subtypecientifico


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