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Abstract
Rotavirus is one of the leading causes of severe acute gastroenteritis in children under 5 years of age, mainly affecting developing countries. Once the disease is acquired, no specific treatment is available; as such, the development of new drugs for effective antirotaviral treatment is critical. Ursolic acid is a pentacyclic triterpenoid with antiviral activity, which has been studied extensively in vitro and in vivo. To study the potential [ver mas...]
dc.contributor.authorTohmé, M. J.
dc.contributor.authorGiménez, M. Cecilia
dc.contributor.authorPeralta, Andrea Veronica
dc.contributor.authorColombo, M. I.
dc.contributor.authorDelgui, Laura Ruth
dc.date.accessioned2020-01-14T14:06:26Z
dc.date.available2020-01-14T14:06:26Z
dc.date.issued2019-11
dc.identifier.issn0924-8579
dc.identifier.otherhttps://doi.org/10.1016/j.ijantimicag.2019.07.015
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6673
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0924857919301955?via%3Dihub#!
dc.description.abstractRotavirus is one of the leading causes of severe acute gastroenteritis in children under 5 years of age, mainly affecting developing countries. Once the disease is acquired, no specific treatment is available; as such, the development of new drugs for effective antirotaviral treatment is critical. Ursolic acid is a pentacyclic triterpenoid with antiviral activity, which has been studied extensively in vitro and in vivo. To study the potential antirotaviral activity of ursolic acid, its toxic potential for viral particles (virucidal effect) and cultured cells (cytotoxicity) was analysed. No effect on virion infectivity was observed with treatments of up to 40 µM ursolic acid, while incipient cytotoxicity started to be evident with 20 µM ursolic acid. The antiviral potential of ursolic acid was evaluated in in-vitro rotavirus infections, demonstrating that 10 µM ursolic acid inhibits rotavirus replication (observed by a decrease in viral titre and the level of the main viral proteins) and affects viral particle maturation (a process associated with the endoplasmic reticulum) 15 h post infection. Interestingly, ursolic acid was also found to hamper the early stages of the viral replication cycle, as a significant reduction in the number and size of viroplasms, consistent with a decrease in VP6 and NSP2 viral proteins, was observed 4 h post infection. As such, these observations demonstrate that ursolic acid exhibits antiviral activity, suggesting that this chemical could be used as a new treatment for rotavirus.eng
dc.formatapplication/pdfeng
dc.language.isoenges_AR
dc.publisherElsevier
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourceInternational journal of antimicrobial agents 54 (5) : 601-609. (Noviembre 2019)eng
dc.subjectRotaviruses_AR
dc.subjectAntiviral Agentseng
dc.subjectViricidases_AR
dc.subjectIn vitroes_AR
dc.subject.otherUrsolic Acideng
dc.subject.otherÁcido Ursólicoes_AR
dc.titleUrsolic acid : a novel antiviral compound inhibiting rotavirus infection in vitroe
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Tohmé, M. J. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Juan Agustín Maza. Facultad de Farmacia y Bioquímica; Argentinaes_AR
dc.description.filFil: Giménez, M. Cecilia. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Juan Agustín Maza. Facultad de Veterinaria y Ciencias Ambientales; Argentinaes_AR
dc.description.filFil: Peralta, Andrea Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Colombo, M. I. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Delgui, Laura Ruth. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentinaes_AR
dc.subtypecientifico


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