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resumen

Resumen
Bovines are the primary reservoir of enterohemorrhagic Escherichia coli (EHEC) O157:H7 and the main source of its transmission to humans. Here, we present a one-year longitudinal study of fecal shedding of E. coli O157. E. coli O157 obtained from recto-anal mucosal samples were characterized by multiplex PCR. The E. coli O157 prevalence ranged from 0.84% in July to 15.25% in November. The confinement within pens resulted in prevalence of 11%. Most [ver mas...]
dc.contributor.authorRhades, Luis
dc.contributor.authorLarzabal, Mariano
dc.contributor.authorBentancor, Adriana
dc.contributor.authorSabio Y Garcia, Julia Veronica
dc.contributor.authorBabinec, Francisco Jose
dc.contributor.authorCataldi, Angel Adrian
dc.contributor.authorAmigo, Natalia
dc.contributor.authorBaldone, Valeria Natalia
dc.contributor.authorUrquiza, Lucas Federico
dc.contributor.authorDelicia, Pablo Javier
dc.contributor.authorFort, Marcelo Cristian
dc.date.accessioned2019-11-20T13:37:50Z
dc.date.available2019-11-20T13:37:50Z
dc.date.issued2019-12-01
dc.identifier.issn0034-5288
dc.identifier.otherhttps://doi.org/10.1016/j.rvsc.2019.10.001
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6345
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0034528819303959
dc.description.abstractBovines are the primary reservoir of enterohemorrhagic Escherichia coli (EHEC) O157:H7 and the main source of its transmission to humans. Here, we present a one-year longitudinal study of fecal shedding of E. coli O157. E. coli O157 obtained from recto-anal mucosal samples were characterized by multiplex PCR. The E. coli O157 prevalence ranged from 0.84% in July to 15.25% in November. The confinement within pens resulted in prevalence of 11%. Most animals (61.86%; 75/118) shed E. coli O157 at least in one sampling occasion. Of the positive animals, 82.19%, 16.44%, and 1.37% were stx positive on one, two and three sampling occasions, respectively. All the E. coli O157 isolated strains carried the genes eae and rfbO157, whereas 11%, 33% and 56% contained stx1, stx2 and stx1/stx2, respectively. The stx1/stx2 and stx2 types were significantly higher during the grazing and finishing periods, respectively, in comparison with the rearing and grazing periods. The presence of stx2a subtype was evident in four isolates, whereas stx2c was present in at least seven. However, both subtypes were present simultaneously in two isolates. The stx1/stx2c, stx1/stx2d and stx1/stx2NT genotypes occurred in 24, 2 and 15 isolates, respectively. The simultaneous occurrence of stx1 and stx2c significantly increased during grazing. Some cases of within-pen and between-pen transmission occurred throughout the study. Contagion levels during in-field grazing were higher than during permanent confinement in the pens. Thus, the individual patterns of shedding varied depending on the proportion of animals shedding the bacteria within pens and the time of shedding.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceResearch in Veterinary Science 127 : 27-32 (December 2019)es_AR
dc.subjectEscherichia colies_AR
dc.subjectVacas de críaes_AR
dc.subjectVirulenciaes_AR
dc.subjectGenéticaes_AR
dc.subjectSuckler Cowseng
dc.subjectGeneticseng
dc.subjectGanado de Carne
dc.subjectBeef Cattleeng
dc.subjectVirulenceeng
dc.titleA one-year longitudinal study of enterohemorrhagic Escherichia coli O157 fecal shedding in a beef cattle herdes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEstación Experimental Agropecuaria Anguiles_AR
dc.description.filFil: Rhades, Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentinaes_AR
dc.description.filFil: Bentancor, Adriana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Larzabal, Mariano.Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Sabio Y Garcia, Julia Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Babinec, Francisco.Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentinaes_AR
dc.description.filFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Amigo, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Baldone, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentinaes_AR
dc.description.filFil: Urquiza, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentinaes_AR
dc.description.filFil: Delicia, Pablo Javier. Universidad Tecnológica Nacional. Facultad Regional Rosario; Argentinaes_AR
dc.description.filFil: Fort, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentinaes_AR
dc.subtypecientifico


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