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Abstract
Cotton blue disease (CBD) is the most important disease present in cotton crops in South America and cotton leafroll dwarf virus (CLRDV) is the causal agent. The disease has been controlled by sowing cotton varieties resistant to CLRDV. However, in the 2009/10 growing season, an outbreak due to an atypical CLRDV isolate (CLRDV‐at) occurred in northwest Argentina. Although CLRDV and CLRDV‐at genomes are very closely related, the symptoms they produce in [ver mas...]
dc.contributor.authorAgrofoglio, Yamila Carla
dc.contributor.authorDelfosse, Veronica Cecilia
dc.contributor.authorCasse, Maria Florencia
dc.contributor.authorHopp, Horacio Esteban
dc.contributor.authorBonacic Kresic, Iván
dc.contributor.authorZiegler-Graff, Véronique
dc.contributor.authorDistefano, Ana Julia
dc.date.accessioned2019-10-29T17:38:26Z
dc.date.available2019-10-29T17:38:26Z
dc.date.issued2019-08
dc.identifier.issn1365-3059
dc.identifier.otherhttps://doi.org/10.1111/ppa.13031
dc.identifier.urihttps://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.13031?af=R
dc.identifier.urihttp://hdl.handle.net/20.500.12123/6232
dc.description.abstractCotton blue disease (CBD) is the most important disease present in cotton crops in South America and cotton leafroll dwarf virus (CLRDV) is the causal agent. The disease has been controlled by sowing cotton varieties resistant to CLRDV. However, in the 2009/10 growing season, an outbreak due to an atypical CLRDV isolate (CLRDV‐at) occurred in northwest Argentina. Although CLRDV and CLRDV‐at genomes are very closely related, the symptoms they produce in cotton plants are quite different. P0 is the most divergent protein between the isolates and in CLRDV is a silencing suppressor protein. This work characterized the silencing suppressor activity of the P0 protein encoded by CLRDV‐at (P0CL‐at) and evaluated its role in Cbd‐resistance break in cotton plants. It was demonstrated that P0CL‐at, despite having a mutation in the consensus of the F‐box‐like motif, was able to suppress local RNA silencing, but displayed lower activity than P0CL. P0CL and P0CL‐at showed no differences in the interaction with Gossypium hirsutum SKP1 orthologue (GSK1) and Nicotiana benthamiana SKP1 and both P0 proteins triggered destabilization of ARGONAUTE1. However, when the ability to enhance PVX symptoms was evaluated, P0CL‐at was shown to be a weaker pathogenicity factor than P0CL in N. benthamiana. Interestingly, trans‐expressed P0CL‐at enabled CLRDV to systemically infect CBD‐resistant plants, and a chimeric CLRDV‐P0CL‐at infectious clone succeeded in establishing infection in CBD‐resistant cotton varieties with symptoms resembling those produced by CLRDV‐at. These results strongly suggest that P0CL‐at is the avirulence (Avr) determinant involved in breaking cotton Cbd gene‐based resistance.es_AR
dc.formatapplication/pdfeng
dc.language.isoeng
dc.publisherWiley
dc.relationinfo:eu-repograntAgreement/INTA/PNBIO/1131023/AR./Prospección y caracterización funcional de genes de interés biotecnológico.es_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesseng
dc.sourcePlant Pathology 68 (6): 1059-1071 (Agosto 2019)es_AR
dc.subjectGossypium Hirsutumes_AR
dc.subjectPoleroviruses_AR
dc.subjectDisease Resistanceeng
dc.subjectResistencia a la Enfermedades_AR
dc.subjectCottoneng
dc.subjectAlgodónes_AR
dc.subject.otherCLRDVeng
dc.subject.otherCotton Leafroll Dwarf Virus
dc.titleP0 protein of cotton leafroll dwarf virus-atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene-based resistancees_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articleeng
dc.typeinfo:eu-repo/semantics/publishedVersioneng
dc.description.origenInstituto de Biotecnologíaes_AR
dc.description.filFil: Agrofoglio, Yamila Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Casse, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentinaes_AR
dc.description.filFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Bonacic Kresic, Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Sáenz Peña; Argentinaes_AR
dc.description.filFil: Ziegler-Graff, Véronique. Université de Strasbourg. Institut de Biologie Moléculaire des Plantes; Franciaes_AR
dc.description.filFil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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